Effect of sevoflurane anesthesia on the comprehensive mRNA expression profile of the mouse hippocampus.
Effect of sevoflurane anesthesia on the comprehensive mRNA expression profile of the mouse hippocampus.
复制标题
七氟醚麻醉对小鼠海马的全面mRNA表达谱的影响。
DOI：
10.4103/2045-9912.184715
复制
发表时间：
2016-04
影响因子：
2.9
通讯作者：
Yamakage M
中科院分区：
文献类型：
--
作者：
Hayase T;Tachibana S;Yamakage M
术后恶心呕吐（PONV）是全麻术后常见的并发症。最近的研究表明，海马参与PONV。假设海马多巴胺能神经元与PONV相关，我们使用七氟烷处理的小鼠模型检查了海马的综合mRNA谱以证实这一点。本研究经我们的机构动物伦理委员会（札幌Medical University School of Medicine的动物研究中心）批准后进行（项目编号：12-033）。将8只小鼠分为两组：未处理组和七氟烷组（Sev组）。在Sev组中，用3.5%七氟烷麻醉4只小鼠1小时。随后，从其海马细胞中分离mRNA，并在Illumina HiSeq 2500平台上进行RNA测序。使用R软件进行质量控制的、过滤的配对末端读数到小鼠基因组的映射和每个基因的表达水平的定量。编码Nogo受体的Rtn 4 rl 2基因是本研究中上调最多的基因。多巴胺受体基因和速激肽基因的表达水平通过七氟烷暴露而增加，而与5-羟色胺受体相关的基因未通过七氟烷暴露而改变。七氟烷高度下调LIM同源结构域相关基因的表达水平。这些结果表明，七氟烷暴露诱导海马神经元的多巴胺能刺激并触发PONV，而由LIM同源结构域相关基因引起的神经元炎症被七氟烷下调。
Postoperative nausea and vomiting (PONV) is a common complication after general anesthesia. Recent studies suggested that the hippocampus is involved in PONV. Hypothesising that hippocampal dopaminergic neurons are related to PONV, we examined the comprehensive mRNA profile of the hippocampus, using a sevoflurane-treated mouse model to confirm this. This study was conducted after approval from our institutional animal ethics committee, the Animal Research Center of Sapporo Medical University School of Medicine (project number: 12-033). Eight mice were assigned to two groups: a naïve group and a sevoflurane group (Sev group). In the Sev group, four mice were anesthetised with 3.5% sevoflurane for 1 hour. Subsequently, mRNA was isolated from their hippocampal cells and RNA sequencing was performed on an Illumina HiSeq 2500 platform. Mapping of the quality-controlled, filtered paired-end reads to mouse genomes and quantification of the expression levels of each gene were performed using R software. The Rtn4rl2 gene that encodes the Nogo receptor was the most up-regulated gene in the present study. The expression levels of dopamine receptor genes and the tachykinin gene were increased by sevoflurane exposure, while the genes related to serotonin receptors were not altered by sevoflurane exposure. The expression levels of LIM-homeodomain-related genes were highly down-regulated by sevoflurane. These findings suggest that sevoflurane exposure induces dopaminergic stimulation of hippocampal neurons and triggers PONV, while neuronal inflammation caused by LIM-homeodomain-related genes is down-regulated by sevoflurane.