Link between embryonic stem cell pluripotency and homologous allelic pairing of Oct4 loci
Link between embryonic stem cell pluripotency and homologous allelic pairing of Oct4 loci
复制标题
DOI：
10.1111/dgd.12403
复制
发表时间：
2017-10
期刊：
影响因子：
4.6
通讯作者：
Masamitsu N. Asaka;Kousuke Uranishi;Ayumu Suzuki;Masataka Hirasaki;M. Nishimoto;A. Okuda
中科院分区：
文献类型：
--
作者：
Masamitsu N. Asaka;Kousuke Uranishi;Ayumu Suzuki;Masataka Hirasaki;M. Nishimoto;A. Okuda
Oct4基因是胚胎干细胞（ESC）多能特性的主要调节因子。最近，Oct4基因座被证明在细胞分化的早期阶段经常定位在彼此接近的位置，这意味着这一事件是ESCs发挥其完全分化潜力的重要先决条件。尽管胚胎癌细胞（ECC）如F9和P19 ECC系的分化能力与ESC相比受到严重限制，但ECC具有与ESC高度相似的表达谱，包括Oct4和其他多能性标记基因的表达。因此，我们研究了Oct4基因座的等位基因配对是否也发生在F9和P19 ECC的分化过程中。我们的数据清楚地表明，这一事件仅在ESC中观察到，而不是ECCs，经历分化诱导，表明Oct4基因座的瞬时等位基因配对是多能ESC的一个特定特征。此外，我们的数据显示，这种配对并没有广泛地发生在携带Oct4基因的17号染色体上，而是发生在Oct4基因座之间，这表明Oct4基因座以某种方式为它们的等位基因配对施加驱动力。
The Oct4 gene is a master regulator of the pluripotent properties of embryonic stem cells (ESCs). Recently, Oct4 loci were shown to frequently localize in close proximity to one another during the early stage of cellular differentiation, implicating this event as an important prerequisite step for ESCs to exert their full differentiation potential. Although the differentiation capacity of embryonal carcinoma cells (ECCs), such as F9 and P19 ECC lines, is severely restricted compared with ESCs, ECCs bear a highly similar expression profile to that of ESCs including expression of Oct4 and other pluripotency marker genes. Therefore, we examined whether allelic pairing of Oct4 loci also occurs during differentiation of F9 and P19 ECCs. Our data clearly demonstrate that this event is only observed within ESCs, but not ECCs, subjected to induction of differentiation, indicating transient allelic pairing of Oct4 loci as a specific feature of pluripotent ESCs. Moreover, our data revealed that this pairing did not occur broadly across chromosome 17, which carries the Oct4 gene, but occurred locally between Oct4 loci, suggesting that Oct4 loci somehow exert a driving force for their allelic pairing.