Identification and characterization of a novel cell cycle-regulated internal ribosome entry site
Identification and characterization of a novel cell cycle-regulated internal ribosome entry site
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DOI：
10.1016/s1097-2765(00)80239-7
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发表时间：
2000-04-01
期刊：
影响因子：
16
通讯作者：
Beyaert, R
中科院分区：
文献类型：
--
作者：
Cornelis, S;Bruynooghe, Y;Beyaert, R
PITSLRE蛋白激酶与细胞周期蛋白依赖性激酶大家族相关。它们被认为可作为肿瘤抑制基因，并已被证明在细胞周期进程中发挥作用。我们报道两种PITSLRE蛋白激酶异构体，即p110（PITSLRE）和p58（PITSLRE），是通过在选择性的读框内AUG密码子起始从单个转录本翻译而来。p110（PITSLRE）由经典的依赖帽子结构的翻译产生，而p58（PITSLRE）则是由具有独特性质的内部核糖体进入位点（IRES）控制的内部翻译起始产生。IRES元件定位于mRNA编码区，其活性受细胞周期调控，这使得p58（PITSLRE）能够在G2/M期进行翻译。
PITSLRE protein kinases are related to the large family of cyclin-dependent kinases. They have been proposed to act as tumor suppressor genes and have been shown to play a role in cell cycle progression. We report that two PITSLRE protein kinase isoforms, namely p110(PITSLRE) and p58(PITSLRE), are translated from a single transcript by initiation at alternative in-frame AUG codons. p110(PITSLRE) is produced by classical cap-dependent translation, whereas p58(PITSLRE) results from internal initiation of translation controlled by an internal ribosome entry site (IRES) with unique properties. The IRES element is localized to the mRNA coding region, and its activity is cell cycle regulated, which permits translation of p58(PITSLRE) in G2/M.