A comparison of exhaled nitric oxide and induced sputum as markers of airway inflammation
A comparison of exhaled nitric oxide and induced sputum as markers of airway inflammation
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DOI：
10.1067/mai.2000.109622
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发表时间：
2000-10-01
影响因子：
14.2
通讯作者：
Hargreave, FE
中科院分区：
文献类型：
--
作者：
Berlyne, GS;Parameswaran, K;Hargreave, FE
背景资料：呼出气一氧化氮（ENO）是哮喘气道炎症的一种无创性标志物。目的：探讨ENO、诱导痰嗜酸性粒细胞（EOS）气道炎症与疾病严重程度生理指标之间的关系（肺功能测定和乙酰甲胆碱PC20），我们还检查了皮质类固醇治疗和特应性对诱导痰中ENO水平和嗜酸性粒细胞计数的影响。在一天内对22名健康的非特应性受试者、28名健康的特应性受试者、38名未服用吸入性类固醇的哮喘受试者、35名服用吸入性类固醇的哮喘受试者和8名患有嗜酸性支气管炎但无哮喘的受试者进行测量。ENO水平与嗜酸性粒细胞分类计数在激素初治哮喘组和健康特应性组中显示出显著但弱的相关性（r（s）< 0.05），与未服用类固醇的哮喘受试者相比，服用类固醇的哮喘受试者中的ENO水平显著较低，尽管痰细胞计数没有差异，并且有增加气流限制的趋势，ENO水平和痰嗜酸性粒细胞计数在与未服用类固醇的哮喘受试者区分方面同样良好。ENO水平不断上升的主题与嗜酸性粒细胞性支气管炎无哮喘，特应性对ENO水平在健康subjects.Conclusion没有影响：我们得出结论，ENO可能有有限的效用作为替代临床测量的存在或嗜酸性粒细胞性气道炎症的严重程度，除了在类固醇治疗的受试者。
Background: Exhaled nitric oxide (ENO) has been proposed as a noninvasive marker of airway inflammation in asthma.Objective: We investigated the relationships among ENO, eosinophilic airway inflammation as measured by induced sputum, and physiologic parameters of disease severity (spirometry and methacholine PC20), We also examined the effect of corticosteroid treatment and atopy on ENO levels and eosinophil counts in induced sputum.Methods: Measurements were taken on one day in 22 healthy nonatopic subjects, 28 healthy atopic subjects, 38 asthmatic subjects not taking inhaled steroids, 35 asthmatic subjects taking inhaled steroids, and 8 subjects with eosinophilic bronchitis without asthma.Results: ENO levels showed significant hut weak correlations with eosinophil differential counts in the steroid-naive asthmatic and healthy atopic groups (r(s) < 0.05), ENO levels were significantly lower in the asthmatic subjects taking steroids compared with the asthmatic subjects not taking steroids, despite there being no difference in the sputum cell counts, and a tendency to increased airflow limitation, ENO levels and sputum eosinophil counts were equally good at differentiating from steroid-naive asthmatic subjects. ENO levels were consistently raised in subjects with eosinophilic bronchitis without asthma, Atopy had no effect on ENO levels in the healthy subjects.Conclusion: We conclude that ENO is likely to have limited utility as a surrogate clinical measurement for either the presence or severity of eosinophilic airway inflammation, except in steroid-naive subjects.