BINDING OF PYROPHOSPHATE AND TWO DIPHOSPHONATES BY HYDROXYAPATITE CRYSTALS
BINDING OF PYROPHOSPHATE AND TWO DIPHOSPHONATES BY HYDROXYAPATITE CRYSTALS
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DOI：
10.1007/bf02547227
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
FLEISCH, H
中科院分区：
文献类型：
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作者：
JUNG, A;BISAZ, S;FLEISCH, H
研究了无机焦磷酸盐(PPJ)与乙烷-1-羟基-1，1-二膦酸二钠(EHDP)和二氯亚甲基二膦酸二钠(Cl2MDP)与羟基磷灰石的结合作用。PPI的结合力最大，EHDP次之，Cl2MDP最小。三者的结合伴随着正磷酸盐向溶液中的释放和晶体对钙的吸收。EHDP和PPI之间存在竞争，EHDP取代PPI的能力比PPI更强。Scatchard图的分析表明，结合并不局限于一类具有相同亲和力的结合位点，第一个结合位点的亲和力顺序为EHDP>；PPI>；Cl2MDP。这些结果与以前关于这些化合物在体外对磷灰石晶体形成和溶解以及对体内骨形成和吸收的影响的数据很好地关联。
The binding on hydroxyapatite has been studied of inorganic pyrophosphate (PPj) and two diphosphonates, disodium ethane-1-hydroxy-1,1-diphosphonate (EHDP) and disodium dichloromethylene diphosphonate (Cl2MDP). The binding is greatest for PPi, less for EHDP and least for Cl2MDP. The binding of all three is accompanied by a release of orthophosphate into the solution and an uptake of calcium by the crystals. A competition exists between EHDP and PPi, EHDP being more potent in displacing PPithan the reverse. An analysis with Scatchard plots suggests that the binding is not restricted to one class of binding sites with equal affinity and that the affinity of the first site is in the order EHDP>PPi>Cl2MDP. The results correlate well with previous data on the effect of these compounds on apatite crystal formation and dissolutionin vitroand on bone formation and resorptionin vivo