Screening of periodontitis with salivary enzyme tests.
Screening of periodontitis with salivary enzyme tests.
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DOI：
10.2334/josnusd.48.177
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发表时间：
2006-12-01
影响因子：
1.9
通讯作者：
Kamoi, Kyuichi
中科院分区：
文献类型：
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作者：
Nomura, Yoshiaki;Tamaki, Yoh;Kamoi, Kyuichi
本研究的目的是确定唾液生化标志物的有用性，用于牙周病的筛查，并检查唾液酶试验的结果之间的协议和探测深度。本研究共纳入187例在日本琦玉县本庄综合保健中心接受年度体检的受试者。用WHO探针进行牙周袋探测，并测量唾液中的各种酶和生化参数。对于乳酸脱氢酶（LDH），计算五种同工酶的比例。为了确定每种酶活性的截止点，构建受试者工作特征曲线（ROC曲线），并确定灵敏度和特异性之间的最小差异点。在检测的生化指标中，唾液LDH水平的敏感性和特异性最高（敏感性0.66，特异性0.67），而唾液天冬氨酸氨基转移酶（AST）和血尿素氮（BUN）水平的敏感性和特异性也在0.60以上。全唾液中LDH同工酶以LDH 4和LDH 5为主。唾液LDH可能是一个可行的和有用的参数，用于牙周病的筛查，而唾液AST和BUN也似乎是潜在的有用的用于此目的。
The purpose of this study was to determine the usefulness of salivary biochemical markers for the screening of periodontal disease and examine the agreement between the results of saliva enzyme tests and those of probing depth. The present study included a total of 187 subjects who underwent annual medical check-ups at the Comprehensive Health Care Center, Honjo, Saitama Prefecture, Japan. Periodontal pocket probing was performed with a WHO probe, and various enzymes and biochemical parameters in saliva were measured. For lactate dehydrogenase (LDH), the proportions of the five isoenzymes were calculated. To decide the cut-off point for each enzymatic activity, receiver operating characteristic curves (ROC curves) were constructed and the points of minimum difference between sensitivity and specificity were decided. Among the biochemical markers tested, salivary LDH level had the highest sensitivity and specificity (sensitivity 0.66, specificity 0.67), while salivary levels of aspartate aminotransferase (AST) and blood urea nitrogen (BUN) also had sensitivity and specificity above 0.60. Among the LDH isoenzymes, LDH4 and LDH5 dominated in whole saliva samples. Salivary LDH may be a feasible and useful parameter for the screening of periodontal disease, while salivary AST and BUN also appear to be potentially useful for this purpose.