Central pupillary light reflex circuits in the cat: I. The olivary pretectal nucleus.
Central pupillary light reflex circuits in the cat: I. The olivary pretectal nucleus.
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猫的中央瞳孔光反射回路：I. 橄榄顶盖前核。
DOI：
10.1002/cne.23602
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
May,PaulJ
中科院分区：
文献类型：
--
作者：
Sun,Wensi;May,PaulJ
通过定义橄榄前叶核（OPt）的视网膜输入、该核的中脑投射和其中的前运动神经元，研究了服务于猫瞳孔光反射的中枢通路。单侧玻璃体内注射小麦胚芽凝集素偶联辣根过氧化物酶（WGA-HRP）显示了两侧视网膜OPt终止模式的差异。将WGA-HRP注射到双侧前正中核的OPt标记终末，少量注射到眼动上区，中央突出Edinger-Westphal核和后联合核。对侧OPt中存在标记的终末细胞和逆行标记的多极细胞，表明存在互交通路。将WGA-HRP注射到前正中核标记的OPt内的梭状前运动神经元，以及后联合核的多极细胞。通过玻璃体室联合前正中核注射WGA-HRP，研究了视网膜终末与前直肠前运动神经元之间的连接。逆行标记的梭状细胞位于逆行标记的视网膜终端区内。超微结构分析显示，标记的视网膜终端区含有清晰的球形囊泡。他们通过不对称的突触密度接触标记的前前额叶前运动神经元。这些结果为猫瞳孔光反射提供了解剖学基础。前部运动前神经元通过暗示兴奋性驱动的突触接受视网膜的直接输入，并直接投射到含有神经节前运动神经元的核。这些突起集中在前正中核，表明其参与瞳孔光反射。中国生物医学工程学报，2014,22(2):379 - 379。©2014 Wiley期刊公司
The central pathways subserving the feline pupillary light reflex were examined by defining retinal input to the olivary pretectal nucleus (OPt), the midbrain projections of this nucleus, and the premotor neurons within it. Unilateral intravitreal wheat germ agglutinin–conjugated horseradish peroxidase (WGA–HRP) injections revealed differences in the pattern of retinal OPt termination on the two sides. Injections of WGA–HRP into OPt labeled terminals bilaterally in the anteromedian nucleus, and to a lesser extent in the supraoculomotor area, centrally projecting Edinger–Westphal nucleus, and nucleus of the posterior commissure. Labeled terminals, as well as retrogradely labeled multipolar cells, were present in the contralateral OPt, indicating a commissural pathway. Injections of WGA–HRP into the anteromedian nucleus labeled fusiform premotor neurons within the OPt, as well as multipolar cells in the nucleus of the posterior commissure. Connections between retinal terminals and the pretectal premotor neurons were characterized by combining vitreous chamber and anteromedian nucleus injections of WGA–HRP in the same animal. Fusiform‐shaped, retrogradely labeled cells fell within the anterogradely labeled retinal terminal field in the OPt. Ultrastructural analysis revealed labeled retinal terminals containing clear spherical vesicles. They contacted labeled pretectal premotor neurons via asymmetric synaptic densities. These results provide an anatomical substrate for the pupillary light reflex in the cat. Pretectal premotor neurons receive direct retinal input via synapses suggestive of an excitatory drive, and project directly to nuclei containing preganglionic motoneurons. These projections are concentrated in the anteromedian nucleus, indicating its involvement in the pupillary light reflex. J. Comp. Neurol. 522:3960–3977, 2014. © 2014 Wiley Periodicals, Inc.