DIFFERENTIAL ALTERATIONS IN PLASMA IL-L AND TNF LEVELS AFTER TRAUMA AND HEMORRHAGE
DIFFERENTIAL ALTERATIONS IN PLASMA IL-L AND TNF LEVELS AFTER TRAUMA AND HEMORRHAGE
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DOI：
10.1152/ajpregu.1991.260.1.r167
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发表时间：
1991-01-01
影响因子：
--
通讯作者：
CHAUDRY, IH
中科院分区：
文献类型：
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作者：
AYALA, A;WANG, P;CHAUDRY, IH
肿瘤坏死因子(TNF)和白介素6(IL-6)是炎症反应的重要介质。目前尚不清楚创伤和出血后这些细胞因子的血浆水平是否会发生变化。我们的目标是确定创伤和出血后是否有血浆肿瘤坏死因子或IL-6的升高，以及这些变化在多大程度上是由于组织创伤而不是单纯出血。SD大鼠在浅乙醚麻醉下，行5 cm正中切开手术，造成创伤。在闭合时，动物被插管，苏醒，出血到平均血压40毫米汞柱，并维持在这个压力，直到最大失血量的40%以乳酸林格液(RL)的形式恢复。然后用相当于失血量四倍的RL复苏动物。分别于失血前、最大失血时(45min)、失血后4h和6h采集血样(0.5ml)获取血浆。用细胞因子依赖的细胞分析法检测IL-6和肿瘤坏死因子水平。肿瘤坏死因子水平在出血后45min显著升高，并持续到出血后4h。IL-6水平在出血后45分钟也升高，并持续到出血后6小时。与肿瘤坏死因子不同，IL-6在中线剖腹手术后和出血开始前已经显著升高，与未经处理的动物相比。因此，创伤对IL-6的诱导可能部分独立于出血时参与释放肿瘤坏死因子的机制。
Tumor necrosis factor (TNF) and interleukin 6 (IL-6) are purported to be important mediators of inflammatory responses. It is not known whether the plasma levels of these cytokines are altered after trauma and hemorrhage. Our objectives were to determine whether there is any elevation of plasma TNF or IL-6 after trauma and hemorrhage and to what extent these changes are due to tissue trauma vs. simple hemorrhage. Trauma was induced in Sprague-Dawley rats under light ether anesthesia by performing a 5-cm midline laparotomy. On closure, animals were catheterized, awakened, hemorrhaged to a mean blood pressure of 40 mmHg, and maintained at that pressure until 40% of maximum shed blood volume was returned in the form of Ringer lactate (RL). Animals were then resuscitated with RL equivalent to four times shed blood volume. Blood samples (0.5 ml) were taken before inducing hemorrhage, at maximal bleed out (45 min), and at 4 and 6 h posthemorrhage to obtain plasma. IL-6 and TNF levels were measured using cytokine-dependent cellular assays. TNF levels were significantly elevated at 45 min into hemorrhage and remained so up to 4 h after hemorrhage. IL-6 levels were also elevated 45 min into hemorrhage and remained so up to 6 h posthemorrhage. IL-6, unlike TNF, was already significantly increased after midline laparotomy and before initiation of hemorrhage compared with unmanipulated animals. Thus induction of IL-6 by trauma may be partially independent of those mechanisms in hemorrhage that are involved in the release of TNF.