Orthosiphon grandiflorum has a protective effect in a calcium oxalate stone forming rat model
Orthosiphon grandiflorum has a protective effect in a calcium oxalate stone forming rat model
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DOI：
10.1007/s00240-010-0265-6
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发表时间：
2010-04-01
影响因子：
--
通讯作者：
Okuyama, Akihiko
中科院分区：
文献类型：
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作者：
Akanae, Wongsawat;Tsujihata, Masao;Okuyama, Akihiko
本研究旨在在动物模型中研究大花正管藻对草酸盐诱导的肾小管细胞损伤的影响以及大花正管藻对尿沉渣形成的抑制作用，并将结果与柠檬酸钾治疗的结果进行比较。将大鼠分为三组：未经处理的成石组、经大花牡丹处理的成石组和经柠檬酸钾处理的成石组。最后一周给大鼠注射乙二醇(0.5%)，并灌服维生素D3(0.5μm)，诱导高草酸尿和肾脏草酸钙结晶沉积。在诱导晶体沉积之前和之后收集二十四小时的尿液样本。三周后处死大鼠并收获两个肾脏。在偏光显微镜下检查平分的肾脏以确定晶体的数量。通过Western blot测定肾组织超氧化物歧化酶和过氧化氢酶水平。通过 8-OHdG 免疫组织荧光检查氧化应激。 O. grandiflorum 和柠檬酸钾具有碱化尿液的能力。在所有组中，与其他组相比，经大花牡丹处理的结石形成组中晶体沉积的数量和8-OHdG染色的水平显着降低。与未经处理的成石组相比，经大花牡丹处理的成石组中的超氧化物歧化酶和过氧化氢酶水平也显着增加。结果表明，大花牡丹对草酸钙结石大鼠模型中的晶体沉积具有显着的抑制作用。
This study amied to investigate the effects of Orthosiphon grandiflorum on the renal tubular cell injury induced by oxalate and the inhibitory effects of O. grandiflorum on urinary deposit formation in an animal model and compared the results with those from a potassium citrate treatment. Rats were divided into three groups: an untreated stone-forming group, an O. grandiflorum-treated stone-forming group and a potassium citrate-treated stone forming group. Ethylene glycol (0.5%) was administered to the rats during the last week, and vitamin D3 (0.5 mu m) was force fed to induce hyperoxaluria and kidney calcium oxalate crystal deposition. Twenty-four hour urine samples were collected before and after inducing crystal deposits. Rats were killed and both kidneys were harvested after 3 weeks. Bisected kidneys were examined under a polarized light microscope to determine the number of crystals. The renal tissue superoxide dismutase and catalase levels were measured by Western blot. Oxidative stress was examined by 8-OHdG immunohistofluorescence. O. grandiflorum and potassium citrate have the ability to alkalinize urine. Among all groups, the number of crystal deposits and the level of 8-OHdG staining decreased significantly in the O. grandiflorum-treated stone forming group, as compared to the other groups. Superoxide dismutase and catalase levels also increased significantly in the O. grandiflorum-treated stone-forming group, as compared with the untreated stone-forming group. The results indicate that O. grandiflorum has a significant inhibitory effect on crystal deposition in the calcium oxalate-stone-forming rat model.