Tissue engineering of nanosilver-embedded peripheral nerve scaffold to repair nerve defects under contamination conditions
Tissue engineering of nanosilver-embedded peripheral nerve scaffold to repair nerve defects under contamination conditions
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纳米银嵌入周围神经支架的组织工程修复污染条件下的神经缺损
DOI：
10.5301/ijao.5000439
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发表时间：
2015-09-01
影响因子：
1.7
通讯作者：
Li, Yun-Qing
中科院分区：
文献类型：
--
作者：
Ding, Tan;Yin, Jun-Bin;Li, Yun-Qing
我们采用纳米银胶原支架，测试其抑制细菌和促进神经再生的效果。方法在前期研究的基础上，制备了不同浓度纳米银的仿生支架，并通过扫描电镜和能谱分析对其内部结构进行了表征。我们将这些支架或自体神经移植物植入大鼠体内修复10毫米的坐骨神经损伤。结果2 mg/ml组对3种细菌培养皿均有bbb10 mm的抑菌带。术后第60天，2 mg/ml组的诱发电位振幅（AMP）和神经传导速度（NCV）均达到最高。2 mg/ml组再生神经更致密、更成熟，髓鞘更厚、排列更整齐。结论纳米银支架（2 mg/ml组）在体外和体内均能有效抑制细菌，降低污染引起的免疫反应，从而促进神经再生和功能恢复。
Introduction We employed a nanosilver-collagen scaffold and tested its effects on inhibiting bacteria and facilitating nerve regeneration. Methods Based on our previous research, we prepared bionic scaffolds with different concentrations of nanosilver and examined their internal structures by scanning electron microscopy and energy dispersive spectroscopy. We implanted these scaffolds or autologous nerve grafts into rats to repair a 10-mm injury of the sciatic nerve. Results The 2 mg/ml group showed a >10 mm bacterial inhibition zone in all 3 types of bacterial culture dishes. At day 60 postsurgery, the 2 mg/ml group also showed the highest amplitude of evoked potential (AMP) and nerve conduction velocity (NCV). The regenerating nerves in the 2 mg/ml group were denser and more mature, and with thicker and well-arrayed myelin sheath. Conclusions These results demonstrate that nanosilver scaffolds (2 mg/ml group) were effective in inhibiting bacteria both in vitro and in vivo, and reduced the contamination-caused immune responses, which in turn promoted nerve regeneration and functional recovery.