Changes in gene body methylation do not correlate with changes in gene expression in Anthozoa or Hexapoda.
Changes in gene body methylation do not correlate with changes in gene expression in Anthozoa or Hexapoda.
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DOI：
10.1186/s12864-022-08474-z
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发表时间：
2022-03-25
期刊：
影响因子：
4.4
通讯作者：
Matz M
中科院分区：
文献类型：
--
作者：
Dixon G;Matz M
随着人类活动改变地球，迫切需要了解生物体如何适应环境变化。该领域越来越感兴趣的是表观遗传修饰（例如 DNA 甲基化）在调整基因表达以适应新条件中的作用。在这里，我们重新分析了九个无脊椎动物（珊瑚虫和六足动物）数据集，以验证这一假设的关键预测：响应某些条件而发生的 DNA 甲基化变化与基因表达的变化相关。根据之前的观察，基因体甲基化（GBM）的基线水平与转录呈正相关，与条件之间的转录变异呈负相关。然而，GBM 变化和转录之间的相关性可以忽略不计。在基因体甲基化类别（高甲基化或低甲基化）水平上，甲基化和转录之间也没有一致的负相关性，正如前面描述的“跷跷板假说”所预期的那样。我们的结果不支持 GBM 直接参与调节无脊椎动物的动态转录反应。如果 DNA 甲基化的变化调节无脊椎动物的转录，那么该机制必须涉及其他因素或调节影响。在线版本包含可在 10.1186/s12864-022-08474-z 获取的补充材料。
As human activity alters the planet, there is a pressing need to understand how organisms adapt to environmental change. Of growing interest in this area is the role of epigenetic modifications, such as DNA methylation, in tailoring gene expression to fit novel conditions. Here, we reanalyzed nine invertebrate (Anthozoa and Hexapoda) datasets to validate a key prediction of this hypothesis: changes in DNA methylation in response to some condition correlate with changes in gene expression. In accord with previous observations, baseline levels of gene body methylation (GBM) positively correlated with transcription, and negatively correlated with transcriptional variation between conditions. Correlations between changes in GBM and transcription, however, were negligible. There was also no consistent negative correlation between methylation and transcription at the level of gene body methylation class (either highly- or lowly-methylated), anticipated under the previously described “seesaw hypothesis”. Our results do not support the direct involvement of GBM in regulating dynamic transcriptional responses in invertebrates. If changes in DNA methylation regulate invertebrate transcription, the mechanism must involve additional factors or regulatory influences. The online version contains supplementary material available at 10.1186/s12864-022-08474-z.
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