Phosphorylation of serine 2 within the RNA polymerase IIC-terminal domain couples transcription and 3′ end processing
Phosphorylation of serine 2 within the RNA polymerase IIC-terminal domain couples transcription and 3′ end processing
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DOI：
10.1016/s1097-2765(03)00492-1
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发表时间：
2004-01-16
期刊：
影响因子：
16
通讯作者：
Buratowski, S
中科院分区：
文献类型：
--
作者：
Ahn, SH;Kim, M;Buratowski, S
RNA聚合酶11的最大亚基含有对转录和mRNA加工偶联重要的独特C末端结构域。该结构域由在丝氨酸2和5处磷酸化的重复七聚体序列（YSPTSPS）组成。丝氨酸5在起始期间被磷酸化并募集加帽酶。丝氨酸2在延伸过程中被Ctk 1激酶磷酸化，Ctk 1激酶是一种类似于哺乳动物Cdk 9/ P-TEFb的蛋白质。染色质免疫沉淀被用来映射的转录延伸和mRNA加工因子在菌株缺乏Ctkl 1的位置。Ctk 1不是延伸因子与转录聚合酶结合所必需的。然而，在ctk 1Delta菌株中，多聚腺苷酸化因子向基因3'区域的募集被破坏，并且观察到3'末端的变化。因此，Ctk 1对丝氨酸2的磷酸化在体内募集了用于共转录3'端加工的因子。
The largest subunit of RNA polymerase 11 contains a unique C-terminal domain important for coupling of transcription and mRNA processing. This domain consists of a repeated heptameric sequence (YSPTSPS) phosphorylated at serines 2 and 5. Serine 5 is phosphorylated during initiation and recruits capping enzyme. Serine 2 is phosphorylated during elongation by the Ctk1 kinase, a protein similar to mammalian Cdk9/ P-TEFb. Chromatin immunoprecipitation was used to map positions of transcription elongation and mRNA processing factors in strains lacking Ctkl1. Ctk1 is not required for association of elongation factors with transcribing polymerase. However, in ctk1Delta strains, the recruitment of polyadenylation factors to 3' regions of genes is disrupted and changes in 3' ends are seen. Therefore, Serine 2 phosphorylation by Ctk1 recruits factors for cotranscriptional 3' end processing in vivo.