Discovery of novel whitefly vector proteins that interact with a virus capsid component mediating virion retention and transmission
Discovery of novel whitefly vector proteins that interact with a virus capsid component mediating virion retention and transmission
复制标题
发现与介导病毒粒子保留和传播的病毒衣壳成分相互作用的新型粉虱载体蛋白
DOI：
10.1016/j.ijbiomac.2022.11.229
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发表时间：
2022-12-29
影响因子：
8.2
通讯作者：
Yan，Feng-Ming
中科院分区：
文献类型：
--
作者：
He，Hai-Fang;Zhao，Chen-Chen;Yan，Feng-Ming
植物病毒传播的特异性和效率在很大程度上取决于载体和病毒之间的蛋白质相互作用。瓜类褪绿黄化病毒（Cucurbit chlorotic yellows virus，CCYV）是由烟粉虱（Bemisia tabaci）以半持久性方式传播的一种病毒，对世界各地的瓜类和蔬菜作物造成严重危害。然而，在CCYV保留和传输过程中的相互作用的分子机制仍然缺乏。CCYV被证明特别结合到粉虱前肠，在这里，我们证实了CCYV的次要外壳蛋白（CPm）参与与载体的相互作用。为了鉴定B.利用免疫沉淀法和DUAL膜cDNA文库筛选技术，筛选了与CCYV的CPm有直接相互作用的烟草花叶病毒（Tabaci）。细胞色素氧化酶亚基5A（考克斯）、微管蛋白β链（TUB）和角蛋白I型细胞骨架9样蛋白（KRT）在B.烟草花叶病毒与CPm有很强的相互作用，与CCYV在载体中的滞留和传播密切相关。这些关于粉虱蛋白-CCYV CPM相互作用的研究结果对于更好地理解昆虫介导的半持久性植物病毒传播机制以及实施有效管理植物病毒及其载体昆虫的新策略至关重要。
Specificity and efficiency of plant virus transmission depend largely on protein-protein interactions of vectors and viruses. Cucurbit chlorotic yellows virus (CCYV), transmitted specifically by tobacco whitefly, Bemisia tabaci, in a semi-persistent manner, has caused serious damage on cucurbit and vegetable crops around the world. However, the molecular mechanism of interaction during CCYV retention and transmission are still lacking. CCYV was proven to bind particularly to the whitefly foregut, and here, we confirmed that the minor coat protein (CPm) of CCYV is participated in the interaction with the vector. In order to identify proteins ofB. tabacithat interact directly with CPm of CCYV, the immunoprecipitation (IP) assay and DUALmembrane cDNA library screening technology were applied. The cytochromecoxidase subunit 5A (COX), tubulin beta chain (TUB) and keratin, type I cytoskeletal 9-like (KRT) ofB. tabacishown strong interactions with CPm and are closely associated with the retention within the vector and transmission of CCYV. These findings on whitefly protein-CCYV CPm interactions are crucial for a much better understanding the mechanism of semi-persistent plant virus transmission by insect vectors, as well as for implement new strategies for effective management of plant viruses and their vector insects.