Protocol for quantifying sound-sensing ability of Drosophila melanogaster
Protocol for quantifying sound-sensing ability of Drosophila melanogaster
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DOI：
10.1038/nprot.2009.206
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发表时间：
2010-01-01
期刊：
影响因子：
14.8
通讯作者：
Ito, Kei
中科院分区：
文献类型：
--
作者：
Inagaki, Hidehiko K.;Kamikouchi, Azusa;Ito, Kei
听觉是大多数动物在交配、寻找食物或抵御猎物时检测声音信号的重要感觉方式。尽管它在许多先天行为中起着关键作用，但关于空气颗粒运动的感觉信息如何在大脑中被检测，处理和整合的知识相对较少。黑腹果蝇具有相当简单的神经系统和大量可用于其研究的分子和遗传工具，是解剖声音感知机制的理想模式生物。在这里，我们描述了测定苍蝇行为的声音反应。虽然这种方法最初是为突变体筛选而开发的，但它也可以与最近的遗传技术相结合，通过沉默或激活选定的神经元集来分析所识别的神经回路的功能。该测定需要类似的15分钟用于实验和1.5小时用于后续分析。
Hearing is an important sensory modality for most animals to detect sound signals as they mate, look for food or fend off prey. Despite its critical role in numerous innate behaviors, relatively little is known about how the sensory information regarding the movement of air particles is detected, processed and integrated in the brain. Drosophila melanogaster, with a rather simple nervous system and the large variety of molecular and genetic tools available for its study, is an ideal model organism for dissecting the mechanisms underlying sound sensing. Here we describe assays to measure sound responses of flies behaviorally. Although this method was originally developed for mutant screening, it can also be combined with recent genetic techniques to analyze functions of the identified neural circuits by silencing or activating select sets of neurons. This assay requires similar to 15 min for an experiment and 1.5 h for subsequent analyses.