CONTACT-DEPENDENT AND GROWTH FACTOR-DEPENDENT SURVIVAL IN A CANINE MARROW-DERIVED STROMAL CELL-LINE
CONTACT-DEPENDENT AND GROWTH FACTOR-DEPENDENT SURVIVAL IN A CANINE MARROW-DERIVED STROMAL CELL-LINE
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DOI：
10.1182/blood.v85.9.2414.bloodjournal8592414
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发表时间：
1995-05-01
期刊：
影响因子：
20.3
通讯作者：
DEEG, HJ
中科院分区：
文献类型：
--
作者：
HUSS, R;HOY, CA;DEEG, HJ
细胞间的相互作用以及干细胞因子(SCF；或c-kit配体)或白介素6(IL-6)等生长因子的存在参与了犬骨髓基质细胞系DO64的增殖和分化。在SCF的存在下，基质细胞被诱导分化，但不增殖。相反，在IL-6存在的情况下，基质细胞在培养中被诱导增殖，而不是分化。干细胞因子和IL-6都是由基质细胞本身产生的，因此可以作为自分泌因子，此外，DO64细胞在最佳培养条件下(细胞融合率为70%~90%，在有血清的情况下)也可以相互作用，从而支持增殖和维持活力。在较低细胞密度或低血清或生长因子浓度的条件下，DO64细胞倾向于聚集并形成簇。这种局部细胞浓度的增加与活性的保存有关，可能是由于自分泌因子的积累。如果没有提供信号，既不是细胞间的，也不是可溶的，DO64细胞不能达到临界的细胞密度，也不能以自分泌的方式产生足够的因子，细胞就会停止增殖，最终死亡。(C)1995年由美国血液病学会主办。
Cell-cell interactions and the presence of growth factors such as stem cell factor (SCF; or c-kit ligand) or interleukin-6 (IL-6) are involved in the proliferation and differentiation of the canine marrow-derived stromal cell line DO64, In the presence of SCF, stromal cells are induced to differentiate, but not to proliferate. In contrast, in the presence of IL-6, stromal cells are induced to proliferate rather than to differentiate in culture. Both SCF and IL-6 are produced by the stromal cells themselves and, thus, act as autocrine factors, In addition, DO64 cells also interact physically with each other in culture when grown under optimal culture conditions (70% to 90% cell confluence and in the presence of serum), thereby supporting proliferation and maintaining viability. Under conditions of lower cell density or low serum or growth factor concentrations in culture, DO64 cells tend to aggregate and form clusters. This increase in local cell concentration is associated with preservation of viability, presumably because of the accumulation of autocrine factors. If no signal, neither intercellular nor soluble, is provided, and DO64 cells are not able to reach a critical cell density or to produce sufficient factors in an autocrine fashion, the cells cease to proliferate and eventually die. (C) 1995 by The American Society of Hematology.