A new pathovar, Pseudomonas syringae pv. alisalensis pv. nov., proposed for the causal agent of bacterial blight of broccoli and broccoli raab
A new pathovar, Pseudomonas syringae pv. alisalensis pv. nov., proposed for the causal agent of bacterial blight of broccoli and broccoli raab
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DOI：
10.1094/pdis.2002.86.9.992
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发表时间：
2002-09-01
期刊：
影响因子：
4.5
通讯作者：
Bull, CT
中科院分区：
文献类型：
--
作者：
Cintas, NA;Koike, ST;Bull, CT
对十字花科蔬菜3种叶部细菌性病害的病原学及其相互关系进行了研究。从LOPAT、碳利用试验和脂肪酸分析的数据表明，青花菜和青花菜的细菌性枯萎病和青花菜的叶斑病是由假单胞菌引起的。从噬菌体敏感性，冰核，单一碳源利用，聚合酶链反应，使用BOXA 1 R引物（BOX-PCR），和主机范围分析的数据是相同的病原体引起叶斑病的青花菜和R rubingae pv.斑点病。西兰花raab和西兰花病原体感染了西兰花raab、所有测试的十字花科植物、番茄和三种单子叶植物（加州雀麦、燕麦和梯牧草）。没有测试的其他病原体（P. moculicola、番茄疫霉致病变种（P. p. coronafaciens）在花椰菜raab上或十字花科植物和单子叶植物上引起疾病。噬菌体敏感性、冰核形成、单一碳源利用、BOX-PCR和寄主范围分析的数据对于来自青花菜raab和青花菜的病原体是相同的，但不同于所测试的其他致病变种，并且支持P.阿利萨勒辛湾nov.引起花椰菜和花椰菜raab上的叶枯病。
The etiology of three foliar bacterial diseases of crucifers and the relationships between their causal agents were evaluated. Data from LOPAT, carbon utilization tests, and fatty acid analysis indicated that bacterial blights of broccoli and broccoli raab, and leaf spot of broccolini, were caused by strains of Pseudomonas syringae. Data from phage sensitivity, ice nucleation, single carbon source utilization, Polymerase chain reaction using BOXA1R primer (BOX-PCR), and host range analyses were identical for the pathogen causing leaf spot of broccolini and R syringae pv. maculicola. The broccoli raab and broccoli pathogens infected broccoli raab, all crucifers tested, tomato, and three monocots (California brome, oat, and common timothy). None of the other pathogens tested (P. syringae pv. moculicola, P. syringae pv.tomato, or P syringae pv. coronafaciens) caused disease on broccoli raab or on both crucifers and monocots. Data from phage sensitivity, ice nucleation, single carbon source utilization, BOX-PCR, and host range analyses were identical for the pathogens from broccoli raab and broccoli, but were different from other pathovars tested, and supported the hypothesis that a new pathovar of P. syringae pv. alisalensis pv. nov. caused a leaf blight on broccoli and broccoli raab.