Alcanivorax hongdengensis sp. nov., an alkane-degrading bacterium isolated from surface seawater of the straits of Malacca and Singapore, producing a lipopeptide as its biosurfactant.
Alcanivorax hongdengensis sp. nov., an alkane-degrading bacterium isolated from surface seawater of the straits of Malacca and Singapore, producing a lipopeptide as its biosurfactant.
复制标题
DOI：
10.1099/ijs.0.001552-0
复制
发表时间：
2009-06
影响因子：
2.8
通讯作者：
Yehui Wu;Qiliang Lai;Zhongwen Zhou;N. Qiao;Chenli Liu;Z. Shao
中科院分区：
文献类型：
--
作者：
Yehui Wu;Qiliang Lai;Zhongwen Zhou;N. Qiao;Chenli Liu;Z. Shao
对从马六甲海峡-新加坡红登码头表层海水中分离到的一株富油菌A-11-3（T）进行了分类学研究。细胞为需氧、革兰氏阴性、不形成孢子的不规则杆状细胞。该菌株为过氧化氢酶和氧化酶阴性。它生长在一个有限的有机化合物光谱，包括一些有机酸和烷烃。16 SrRNA基因序列分析表明，菌株A-11-3（T）与模式菌株Jadensis（96.8%）、Borhanovorax（96.8%）、Alcanivorax dieselolei（94.8%）、Alcanivorax venustensis（94.2%）和Alcanivorax balearicus（94.0%）的亲缘关系最近。主要脂肪酸为C（16：0）（31.2%）、C（18：1）ω 7 c（24.8%）、C（18：0）（9.6%）、C（12：0）（8.3%）、C（16：1）ω 7 c（8.3%）和C（16：0）3-OH（5.1%）。基因组DNA的G+C含量为54.7mol%。此外，该菌株产生脂肽作为其表面活性化合物。根据生理生化试验、DNA-DNA杂交结果以及16 S-23 S内转录间隔区、gyrB基因和烷烃羟化酶基因alkB 1的序列比较，菌株A-11-3（T）归属于食烷菌属，但与已知的食烷菌属物种很容易区分。因此，菌株A-11-3（T）代表食烷菌属的一个新种，并建议将其命名为洪登食烷菌（Alcanivorax hongdengensis sp. nov.）。模式菌株为A-11-3（T）（=CGMCC 1.7084（T）=LMG 24624（T）=MCCC 1A 01496（T））。
A taxonomic study was carried out on strain A-11-3(T), which was isolated from an oil-enriched consortia from the surface seawater of Hong-Deng dock in the Straits of Malacca and Singapore. Cells were aerobic, Gram-negative, non-spore-forming irregular rods. The strain was catalase- and oxidase-negative. It grew on a restricted spectrum of organic compounds, including some organic acids and alkanes. 16S rRNA gene sequence comparisons showed that strain A-11-3(T) was most closely related to the type strains of Alcanivorax jadensis (96.8 % sequence similarity), Alcanivorax borkumensis (96.8 %), Alcanivorax dieselolei (94.8 %), Alcanivorax venustensis (94.2 %) and Alcanivorax balearicus (94.0 %). The predominant fatty acids were C(16 : 0) (31.2 %), C(18 : 1)omega7c (24.8 %), C(18 : 0) (9.6 %), C(12 : 0) (8.3 %), C(16 : 1)omega7c (8.3 %) and C(16 : 0) 3-OH (5.1 %). The G+C content of the genomic DNA was 54.7 mol%. Moreover, the strain produced lipopeptides as its surface-active compounds. According to physiological and biochemical tests, DNA-DNA hybridization results and sequence comparisons of the 16S-23S internal transcribed spacer, the gyrB gene and the alkane hydroxylase gene alkB1, strain A-11-3(T) was affiliated with the genus Alcanivorax but could be readily distinguished from recognized Alcanivorax species. Therefore strain A-11-3(T) represents a novel species of the genus Alcanivorax for which the name Alcanivorax hongdengensis sp. nov. is proposed. The type strain is A-11-3(T) (=CGMCC 1.7084(T)=LMG 24624(T)=MCCC 1A01496(T)).