Self-assembly of cationic multidomain peptide hydrogels: supramolecular nanostructure and rheological properties dictate antimicrobial activity.
Self-assembly of cationic multidomain peptide hydrogels: supramolecular nanostructure and rheological properties dictate antimicrobial activity.
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DOI：
10.1039/c5nr05233e
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发表时间：
2015-12-07
期刊：
影响因子：
6.7
通讯作者：
Dong H
中科院分区：
文献类型：
--
作者：
Jiang L;Xu D;Sellati TJ;Dong H
水凝胶是一类重要的生物材料，已广泛用于各种生物医学/医学应用。如果水凝胶具有固有的抗菌活性，能够避免机会性细菌病原体在伤口部位的定植，那么水凝胶的生物学性能，特别是用作伤口敷料的水凝胶，可以大大提高。拥有这种抗菌特性还可以保护水凝胶本身免受附着在其表面的微生物的不利影响。我们之前已经证明了溶液中阳离子多域肽（MDP）超分子组装体的广谱抗菌活性。在这里，我们将 1-D 可溶性超分子组装扩展到 3-D 水凝胶，以研究超分子纳米结构及其流变特性对自组装水凝胶抗菌活性的影响。在设计的 MDP 中，发现肽水凝胶的杀菌活性与溶液中的杀菌活性相反。自组装肽水凝胶的抗菌活性的提高是由超分子表面化学和本体材料的储能模量的综合效应决定的，而不是由溶液中观察到的单个肽/肽组装体穿透细菌细胞膜的能力决定的。通过这项研究开发的结构-性质-活性关系将为设计具有内置抗菌活性的生物相容性肽水凝胶用于各种生物医学应用提供重要指导。
Hydrogels are an important class of biomaterials that have been widely utilized for a variety of biomedical/medical applications. The biological performance of hydrogels, particularly those used as wound dressing could be greatly advanced if imbued with inherent antimicrobial activity capable of staving off colonization of the wound site by opportunistic bacterial pathogens. Possessing such antimicrobial properties would also protect the hydrogel itself from being adversely affected by microbial attachment to its surface. We have previously demonstrated the broad-spectrum antimicrobial activity of supramolecular assemblies of cationic multi-domain peptides (MDPs) in solution. Here, we extend the 1-D soluble supramolecular assembly to 3-D hydrogels to investigate the effect of the supramolecular nanostructure and its rheological properties on the antimicrobial activity of self-assembled hydrogels. Among designed MDPs, the bactericidal activity of peptide hydrogels was found to follow an opposite trend to that in solution. Improved antimicrobial activity of self-assembled peptide hydrogels is dictated by the combined effect of supramolecular surface chemistry and storage modulus of the bulk materials, rather than the ability of individual peptides/peptide assemblies to penetrate bacterial cell membrane as observed in solution. The structure–property–activity relationship developed through this study will provide important guidelines for designing biocompatible peptide hydrogels with built-in antimicrobial activity for various biomedical applications.