DESIGN OF A HIGH-PRECISION FRACTION COLLECTOR FOR CAPILLARY ELECTROPHORESIS
DESIGN OF A HIGH-PRECISION FRACTION COLLECTOR FOR CAPILLARY ELECTROPHORESIS
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DOI：
10.1021/ac00113a036
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发表时间：
1995-09-01
影响因子：
7.4
通讯作者：
KARGER, BL
中科院分区：
文献类型：
--
作者：
MULLER, O;FORET, F;KARGER, BL
研制了一种高精度毛细管电泳级分收集器。该装置利用靠近毛细管末端的检测和毛细管出口处的鞘液体，允许连续收集(即，不间断的外加电场)多种物种，评估了鞘液体在柱中的检测速度和位置对收集精度的作用。在毛细管出口前约1厘米处的光纤检测对于收集的精确计时是有效的。多达60微升或更小的体积可以自动收集到用作收集瓶的毛细管中。收集毛细管放置在圆柱体上，计算机控制的步进电机将适当的毛细管与柱出口对准。该片段收集器在收集Phi X-174质粒DNA的全部11个HaeIII限制性内切酶片段中得到了验证。聚合酶链式反应扩增271和281bp片段显示大小依赖的迁移顺序倒置。
high-precision fraction collector for capillary electrophoresis has been developed. The device utilizes detection close to the end of the capillary and a sheath liquid at the exit of the capillary, allowing continuous collection (i.e., uninterrupted applied electrical field) of multiple species, The role of the sheath liquid now rate and position of detection in the column on the collection precision was assessed. Fiber-optic detection at similar to 1 cm before the exit end of the capillary was found effective for precise timing of the collection. Up to 60 fractions of microliter or smaller volumes could be automatically collected into capillaries used as collection vials. The collection capillaries were placed on a cylinder, and a computer-controlled stepping motor aligned the appropriate capillary with the column exit. The effectiveness of the fraction collector was demonstrated in the collection of all 11 fragments of the HaeIII restriction digest of Phi X-174 plasmid DNA. Polymerase chain reaction amplification of the 271 and 281 bp fragments revealed an inversion of the size-dependent migration order.