Long-term infection of mongolian gerbils with Helicobacter pylori:: Microbiological, histopathological, and serological analyses
Long-term infection of mongolian gerbils with Helicobacter pylori:: Microbiological, histopathological, and serological analyses
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DOI：
10.1128/cdli.12.2.347-353.2005
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发表时间：
2005-02-01
期刊：
影响因子：
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通讯作者：
Kamiya, S
中科院分区：
文献类型：
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作者：
Nakagawa, S;Osaki, T;Kamiya, S
本文观察了幽门螺杆菌长期感染对蒙古沙土鼠胃粘膜的影响。H.的定殖pylori通过微需氧培养和实时逆转录酶PCR（RT-PCR）进行评估。持续感染H.感染后6个月内胃粘膜组织用实时荧光定量RT-PCR法检测到pylori，但未检测到H.感染后4个月通过培养分离pylori。从感染后2个月开始观察到中性粒细胞和单核细胞浸润。肠上皮化生和胃萎缩也从感染后2个月开始检测。酶联免疫吸附试验结果表明，抗全H。pylori抗原、H.幽门螺杆菌热休克蛋白60（HSP 60）和大肠杆菌GroEL在感染的沙鼠中显著高于未感染的沙鼠。长期感染H. pylori感染18个月后，腺胃粘膜明显萎缩，粘膜下层可见多发囊肿。此外，所有感染沙鼠的贲门均可见角化过度的鳞状细胞乳头状瘤。这些结果表明，在长期感染的细菌定植的评估可以通过实时RT-PCR和粘膜损伤可能是由宿主免疫反应，对整个H。pylori抗原
The effects of long-term infection with Helicobacter pylori on the gastric mucosa of Mongolian gerbils were examined. Colonization by H. pylori was evaluated by both microaerobic cultivation and real-time reverse transcriptase PCR (RT-PCR). Persistent infection with H. pylori in gastric mucosa was detected by real-time RT-PCR during 6 months after infection, but no H. pylori was isolated 4 months after infection by cultivation. Infiltration with neutrophils and mononuclear cells was observed from 2 months after infection. Both intestinal metaplasia and gastric atrophy were also detected from 2 months after infection. The results by enzyme-linked immunosorbent assay indicated that antibody titers against whole H. pylori antigens, H. pylori heat shock protein 60 (HSP60), and Escherichia coli GroEL were significantly higher in the infected gerbils than in noninfected gerbils. After long-term infection with H. pylori for 18 months, marked atrophy of gastric mucosa and multiple cysts in the submucosa were observed in the glandular stomach of the infected gerbils. In addition, squamous cell papilloma with hyperkeratosis was observed in cardia of all the infected gerbils. These results indicate that evaluation of bacterial colonization during long-term infection can be done by real-time RT-PCR and that mucosal damage might be induced by host immune response against whole H. pylori antigen.