Expression of Mn-superoxide dismutase gene in nontumorigenic and tumorigenic human mammary epithelial cells
Expression of Mn-superoxide dismutase gene in nontumorigenic and tumorigenic human mammary epithelial cells
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DOI：
10.1155/s1110724304401016
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发表时间：
2004-09-30
影响因子：
--
通讯作者：
Mukherjee, S
中科院分区：
文献类型：
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作者：
Mukhopadhyay, S;Das, SK;Mukherjee, S
锰超氧化物歧化酶位于线粒体基质中，具有保护细胞免受氧化损伤的功能。已有报道称，低水平的Mn-SOD基因表达会导致某些肿瘤的发生。另一方面，锰-超氧化物歧化酶基因的过度表达可能在肿瘤的发生发展中起重要作用。在我们的研究中，我们发现非侵袭性(MCF-7)和侵袭性(BT-549和11-9-14)乳腺癌细胞株的Mn-SOD活性高于非致瘤性乳腺癌细胞系(MCF-12A和MCF-12F)。我们还观察到癌细胞系中Mn-SOD基因的表达增加。非侵袭性和侵袭性乳腺上皮细胞系中超氧化物歧化酶活性的升高与核苷酸序列的某些改变有关。
Manganese superoxide dismutase (Mn-SOD), localized at the mitochondrial matrix, has the ability to protect cells against oxidative damage. It has been reported that low levels of Mn-SOD gene expression cause the development of certain kind of tumors. On the other hand, overexpression of Mn-SOD gene may play an important role in the development of cancer. In our study, we find that Mn-SOD activity was higher in nonaggressive (MCF-7) and aggressive (BT-549 and 11-9-14) breast cancer cell lines compared to that of nontumorigenic (MCF-12A and MCF-12F) mammary epithelial cell lines. We also observed an increased expression of Mn-SOD gene in cancerous cell lines. The elevated level of SOD activity in nonaggressive and aggressive breast epithelial cell lines was associated with some changes in nucleotide sequence.