Parallel trapping of single motile cells based on vibration-induced flow
Parallel trapping of single motile cells based on vibration-induced flow
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DOI：
10.1007/s10404-018-2062-4
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发表时间：
2018-04-01
影响因子：
2.8
通讯作者：
Arai, Fumihito
中科院分区：
文献类型：
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作者：
Hayakawa, Takeshi;Akita, Yusuke;Arai, Fumihito
我们提出了一种芯片上的细胞操作方法来并行捕获单个运动细胞。所提出的方法捕获大型（大于或类似于50 μ m）的运动细胞，这是传统的基于光学、声学、电或磁力的细胞操作方法难以实现的。该捕获方法利用对表面有微结构的微流控芯片施加振动所引起的流动。通过对带有微柱对的芯片施加直线振动，我们可以在微柱之间产生的局部流动中捕获单个运动细胞。利用所提出的方法，我们平行捕获单个细叶茅细胞（大小为50-100 μ m）。此外，我们还评估了不同微柱阵列设计参数的捕集性能以及通过改变振动幅值来控制捕集流速的可控性。然后在运动细胞的运动性评估中证明了所提出的方法。该演示证实了所提出的方法在实现单个运动细胞的高通量运动评估方面的潜力。
We propose an on-chip cell manipulation method for trapping single motile cells in parallel. The proposed method traps large (greater than or similar to 50 mu m) motile cells in parallel, which is difficult to achieve by conventional cell manipulation methods based on optical, acoustic, electric, or magnetic forces. The trapping method exploits the flow induced by applying a vibration to a microfluidic chip with microstructures on its surface. By applying a rectilinear vibration to a chip with pairs of micropillars, we can trap single motile cells within the local flow generated between the micropillars. Using the proposed method, we trapped single Euglena gracilis cells (of size 50-100 mu m) in parallel. Moreover, we evaluate the trapping performance for various micropillar array design parameters and the controllability of the trapping-flow velocity by varying the amplitude of the vibration. The proposed method was then demonstrated in a motility evaluation of motile cells. The demonstration confirms the potential of the proposed method in realizing high-throughput motility evaluations of single motile cells.