Ubiquitinated alpha B-crystallin in glial cytoplasmic inclusions from the brain of a patient with multiple system atrophy.
Ubiquitinated alpha B-crystallin in glial cytoplasmic inclusions from the brain of a patient with multiple system atrophy.
复制标题
多系统萎缩患者大脑胶质细胞质内含物中的泛素化 α B 晶状体蛋白。
DOI：
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发表时间：
1995
影响因子：
4.4
通讯作者：
S. Shoji
中科院分区：
文献类型：
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作者：
A. Tamaoka;H. Mizusawa;H. Mori;S. Shoji
在少突胶质细胞样细胞中观察到胶质细胞胞质内含物（GCI），特别是在多系统萎缩（纹状体黑质变性、橄榄脑桥小脑萎缩和Shy-Drager综合征）患者的脑中。我们用生物化学和免疫化学方法研究了多系统萎缩患者大脑的GCI。虽然大多数GCI在免疫细胞化学研究中报告为泛素和α B-晶状体蛋白阳性，但GCI的组分尚未进行生物化学鉴定。通过用尼龙网筛和不连续蔗糖密度梯度离心从OPCA脑中部分纯化GCI-承载细胞。还发现含有GCI-承载细胞的级分含有32 kDa和40 kDa蛋白质，这两种蛋白质都被抗-泛素和抗-α B-晶状体蛋白抗体特异性识别，这两种抗体都未在来自对照脑的相同级分中发现。这些免疫化学结果表明，泛素化的α B-晶体蛋白存在于GCI从多系统萎缩患者的大脑。
Glial cytoplasmic inclusions (GCIs) have been observed in oligodendroglia-like cells, specifically in the brains of patients with multiple system atrophy (striatonigral degeneration, olivopontocerebellar atrophy and Shy-Drager syndrome). We have investigated GCIs from brains of patients with multiple system atrophy biochemically and immunochemically. While most GCIs have been reported positive for both ubiquitin and alpha B-crystallin in immunocytochemical studies, the components of GCIs have not been identified biochemically. GCI-bearing cells were partially purified from the OPCA brain by sieving with nylon meshes and discontinuous sucrose density gradient centrifugation. The fraction containing GCI-bearing cells was also found to contain a 32 kDa and a 40 kDa protein, both of which were specifically recognized by anti-ubiquitin and anti-alpha B-crystallin antibodies, neither of which was found in the same fraction derived from control brain. These immunochemical results suggest that ubiquitinated alpha B-crystallin is present in GCIs from the brains of patients with multiple system atrophy.