Apoptosis induction by Epican Forte in HTLV-1 positive and negative malignant T-cells
Apoptosis induction by Epican Forte in HTLV-1 positive and negative malignant T-cells
复制标题
DOI：
10.1016/j.leukres.2005.11.027
复制
发表时间：
2006-07-01
影响因子：
2.7
通讯作者：
Rath, M.
中科院分区：
文献类型：
--
作者：
Harakeh, S.;Diab-Assaf, M.;Rath, M.
使用针对 HTLV-1 阳性（HuT-102 和 C91-PL）和阴性（CEM 和 Jurkat）细胞的非细胞毒性浓度，评估新型营养制剂 Epican Forte (EF) 对增殖和诱导细胞凋亡的影响。通过 MTT 测定和使用 RTPCR 的 TGF mRNA 蛋白表达测定，EF 显示出抗增殖作用。 EF导致TGF-α下调和TGF-β2上调。EF导致preG(1)期凋亡细胞显着增加。使用细胞死亡 ELISA 和膜联蛋白 V-FITC 证实了这些结果。细胞凋亡的诱导是由 p53、p21 和 Bax 蛋白水平上调以及 Bcl-2 α 蛋白表达水平下调引起的。 (c) 2005 Elsevier Ltd. 保留所有权利。
The effects of a novel nutrient formulation Epican Forte (EF) were evaluated on proliferation and induction of apoptosis using non-cytotoxic concentrations against HTLV-1 positive (HuT-102 & C91-PL) and negative (CEM & Jurkat) cells.EF showed anti-proliferative effect as determined by MTT assay and TGF mRNA protein expression using RTPCR. EF resulted in the down-regulation of TGF-alpha and an up-regulation in TGF-beta 2.EF caused a significant increase in apoptotic cells in the preG(1) phase. These results were confirmed using Cell Death ELISA and Annexin V-FITC. Induction of apoptosis was caused by an up-regulation of p53, p21 and Bax protein levels and a down-regulation of Bcl-2 alpha protein expression level. (c) 2005 Elsevier Ltd. All rights reserved.