Transcriptomic analysis of Portunus trituberculatus reveals a critical role for WNT4 and WNT signalling in limb regeneration
Transcriptomic analysis of Portunus trituberculatus reveals a critical role for WNT4 and WNT signalling in limb regeneration
复制标题
三疣梭子蟹的转录组分析揭示了 WNT4 和 WNT 信号在肢体再生中的关键作用
DOI：
10.1016/j.gene.2018.03.015
复制
发表时间：
2018
期刊：
影响因子：
3.5
通讯作者：
Chunlin Wang
中科院分区：
文献类型：
--
作者：
Lei Liu;Yuanyuan Fu;Fang Zhu;Changkao Mu;Ronghua Li;Weiwei Song;Ce Shi;Yangfang Ye;Chunlin Wang
游蟹（Portunus trituberculatus）是亚洲最具经济价值的海水甲壳类动物之一。尽管这种螃蟹具有重要的商业价值和被充分研究的地位，但这种螃蟹的基因组和转录组学数据很少。本研究收集截肢后不同发育阶段的肢体芽组织进行转录组学分析。应用Illumina rna测序对肢体再生转录组进行表征，并鉴定出最具特征的基因。通过对clean reads的聚类和组装，共获得289,018个转录本，产生150,869个unigenes，平均长度为956 bp。随后的分析显示WNT信号是参与肢体再生的关键途径，WNT4是一个关键的中介。总体而言，肢体再生似乎受到多种信号通路的调控，包括WNT4、LAMA、FIP2、FSTL5、TNC、HUS1、SWI5、NCGL、SLC22、PLA2、Tdc2、SMOX、GDH和SMPD4在内的许多细胞分化、肌肉生长、脱毛、代谢和免疫相关基因上调。这是第一次进行爪爪再生的实验研究。trituberculatus。这些发现扩大了现有的螃蟹物种序列资源，并可能加速甲壳类动物再生和发育的研究，特别是对涉及肢体再生的基因的功能研究。
The swimming crab (Portunus trituberculatus) is among the most economically important seawater crustacean species in Asia. Despite its commercial importance and being well-studied status, genomic and transcriptomic data are scarce for this crab species. In the present study, limb bud tissue was collected at different developmental stages post amputation for transcriptomic analysis. Illumina RNA-sequencing was applied to characterise the limb regeneration transcriptome and identify the most characteristic genes. A total of 289,018 transcripts were obtained by clustering and assembly of clean reads, producing 150,869 unigenes with an average length of 956 bp. Subsequent analysis revealed WNT signalling as the key pathway involved in limb regeneration, with WNT4 a key mediator. Overall, limb regeneration appears to be regulated by multiple signalling pathways, with numerous cell differentiation, muscle growth, moult, metabolism, and immune-related genes upregulated, including WNT4, LAMA, FIP2, FSTL5, TNC, HUS1, SWI5, NCGL, SLC22, PLA2, Tdc2, SMOX, GDH, and SMPD4. This is the first experimental study done on regenerating claws ofP. trituberculatus. These findings expand existing sequence resources for crab species, and will likely accelerate research into regeneration and development in crustaceans, particularly functional studies on genes involved in limb regeneration.