Biosensors from conjugated polyelectrolyte complexes
Biosensors from conjugated polyelectrolyte complexes
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DOI：
10.1073/pnas.012581399
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发表时间：
2002-01-08
影响因子：
11.1
通讯作者：
Heeger, AJ
中科院分区：
文献类型：
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作者：
Wang, DL;Gong, X;Heeger, AJ
通过将发射橙子光的阴离子共轭双链体和饱和阳离子双链体以1：1的比例（每个重复单元）组合，在水溶液中形成电荷中性络合物（CNC）。CNC的光致发光（PL）可以被带负电荷的二硝基苯酚（DNP）衍生物（DNP-BS-）和带正电荷的甲基紫精（MV 2+）猝灭。CNC的使用最大限度地减少了共轭聚电解质和生物聚合物之间的非特异性相互作用（修改PL）。观察到DNP衍生物对来自CNC的PL的淬灭和加入抗DNP抗体（抗DNP IgG）时的特异性非淬灭。因此，证明了抗DNP IgG的生物传感。
A charge neutral complex (CNC) was formed in aqueous solution by combining an orange light emitting anionic conjugated polyelectrolyte and a saturated cationic polyelectrolyte at a 1:1 ratio (per repeat unit). Photoluminescence (PL) from the CNC can be quenched by both the negatively charged dinitrophenol (DNP) derivative, (DNP-BS-), and positively charged methyl viologen (MV2+). Use of the CNC minimizes nonspecific interactions (which modify the PL) between conjugated polyelectrolytes and biopolymers. Quenching of the PL from the CNC by the DNP derivative and specific unquenching on addition of anti-DNP antibody (anti-DNP IgG) were observed. Thus, biosensing of the anti-DNP IgG was demonstrated.