MRC image processing programs
MRC image processing programs
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DOI：
10.1006/jsbi.1996.0003
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发表时间：
1996-01-01
影响因子：
3
通讯作者：
Smith, JM
中科院分区：
文献类型：
--
作者：
Crowther, RA;Henderson, R;Smith, JM
数字图像处理是通过电子显微镜测定大分子结构的重要步骤。集中重要的程序是亚基的许多图像的平均，以改善信号，对各种传输函数的校正以及从一组二维预测的三维图生成。最好执行这些计算过程的详细方式取决于对象的对称性和样品的类型。多年来，分子生物学实验室的各个成员已经编写了一系列程序，用于处理二维晶体和具有螺旋形或二十面体对称性的颗粒的图像。该理念是编写独立程序，并且通过采用标准格式来存储和互换不同类型的数据，整个系统具有连贯性。本文描述了程序的当前状态。 （c）1996 Academic Press，Inc。
Digital image processing is an essential step in the determination of macromolecular structures by electron microscopy. Centrally important procedures are the averaging of many images of the subunit to improve the signal, the correction for various transfer functions, and the generation of a three-dimensional map from a set of two-dimensional projections. The detailed way in which these computational procedures are best carried out depends on the symmetry of the object and the type of specimen preparation. Over many years a large set of programs has been written by various members of the Laboratory of Molecular Biology for processing images of two-dimensional crystals and of particles with helical or icosahedral symmetry. The philosophy has been to write stand-alone programs and the whole system is given coherence by the adoption of standard formats for the storage and interchange of different kinds of data. This paper describes the current state of the programs. (C) 1996 Academic Press, Inc.