CHARACTERIZATION OF A RICE (ORYZA-SATIVA L) MUTANT DEFICIENT IN THE HEME DOMAIN OF NITRATE REDUCTASE
CHARACTERIZATION OF A RICE (ORYZA-SATIVA L) MUTANT DEFICIENT IN THE HEME DOMAIN OF NITRATE REDUCTASE
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DOI：
10.1007/bf00223974
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发表时间：
1992-06-01
影响因子：
5.4
通讯作者：
ICHII, M
中科院分区：
文献类型：
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作者：
HASEGAWA, H;KATAGIRI, T;ICHII, M
对水稻硝酸还原酶（NR）缺失突变体M819进行了生化和遗传鉴定。M819叶片NADH-NR活性约为野生型cv ‘Norin 8’的10%，NADPH-NR活性高于野生型；FMNH2-NR和MV-NR活性为野生型的10%；BPB-NR活性高于野生型；黄嘌呤脱氢酶活性在两者中均存在。这些结果表明，突变系M819由于结构基因编码区域的点突变或小缺失而缺乏NADH-NR多肽的功能性血红素结构域。M819的氯酸盐抗性是通过单隐性核基因遗传的。
Biochemical and genetical characterization of a rice nitrate reductase (NR)-deficient mutant, M819, which had been isolated as a chlorate-resistant mutant, was carried out. In M819, leaf NADH-NR activity was found to be about 10% of that of the wild-type cv 'Norin 8', while NADPH-NR activity was higher than that in the wild-type; FMNH2-NR and MV-NR activities were also 10% of those of the wild type; BPB-NR activity was higher than that of the wild type; and xanthine dehydrogenase activity was revealed to be present in both. These results suggest that the mutant line M819 lacks the functional heme domain of the NADH-NR polypeptide due to a point mutation or a small deletion within the coding region of the structural gene. Chlorate resistance in M819 was transmitted by a single recessive nuclear gene.