Expression of cell-surface antigens on rat colonic cancer cells.
Expression of cell-surface antigens on rat colonic cancer cells.
复制标题
细胞表面抗原在大鼠结肠癌细胞上的表达。
DOI：
10.1016/0016-5085(88)90420-9
复制
发表时间：
1988
期刊：
影响因子：
29.4
通讯作者：
Ahnen,DJ
中科院分区：
文献类型：
--
作者：
Sugiyama,T;Brown,WR;Ahnen,DJ
以前，我们报道了两种糖蛋白，癌胚抗原和分泌成分，分别优先表达在正常人结肠上皮细胞的顶端或侧基底表面，而这些抗原可能在结肠癌细胞的整个细胞表面表达。这些观察结果促使我们建立了结肠癌中异常分布的正常表面抗原的动物模型。我们制备了四种单克隆抗体，其中三种与位于微绒毛表面的抗原特异性反应，一种与正常大鼠结肠细胞的侧基底表面特异性反应。四种细胞表面抗原中的三种在结肠隐窝的所有水平上由细胞表达，但其中一种微绒毛抗原（p66）仅在管腔表面的成熟结肠细胞上表达。然后，我们记录了一个微绒毛抗原（p66）检测的抗体之一和侧基底膜抗原检测的另一种抗体在整个表面的低分化结肠癌细胞诱导二甲基肼。p66在低分化结肠癌中的表达率高于其他三种抗原。这些观察结果表明：（a）表面膜抗原的极性是正常大鼠结肠上皮细胞的一个特征，（B）这种极性在某些实验性结肠癌中不存在，（c）结肠癌细胞的恶性表型不仅仅是由于结肠细胞正常分化的阻断。
Previously, we reported that two glycoproteins, carcinoembryonic antigen and secretory component, are preferentially expressed on the apical or laterobasal surfaces, respectively, of normal human colonic epithelial cells, whereas these antigens may be expressed over the entire cell surface of colonic cancer cells. These observations have prompted us to develop an animal model of aberrantly distributed normal surface antigens in colonic carcinoma. We prepared four monoclonal antibodies, three of which react specifically with antigens located on the microvillus surface and one that reacts specifically with the laterobasal surface of normal rat colonocytes. Three of the four cell-surface antigens were expressed by cells at all levels of the colonic crypt, but one of the microvillar antigens (p66) was expressed only on the mature colonocytes at the luminal surface. We then documented that a microvillus antigen (p66) detected by one of the antibodies and a laterobasal membrane antigen detected by another antibody were expressed over the entire surface of poorly differentiated colonic carcinoma cells induced by dimethylhydrazine. p66 was more often expressed on poorly differentiated colonic cancers than were the other three antigens. These observations indicate that (a) polarity of surface membrane antigens is a characteristic of normal rat colonic epithelial cells, (b) this polarity is not present in some experimental colonic carcinomas, and (c) the malignant phenotype of colonic carcinoma cells does not result merely from a block in normal differentiation of colonocytes.