ANDROGEN ENHANCES NEURONAL DEGENERATION IN THE DEVELOPING PREOPTIC AREA - APOPTOSIS IN THE ANTEROVENTRAL PERIVENTRICULAR NUCLEUS (AVPVN-POA)
ANDROGEN ENHANCES NEURONAL DEGENERATION IN THE DEVELOPING PREOPTIC AREA - APOPTOSIS IN THE ANTEROVENTRAL PERIVENTRICULAR NUCLEUS (AVPVN-POA)
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DOI：
10.1006/hbeh.1994.1027
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发表时间：
1994-12-01
影响因子：
3.5
通讯作者：
NISHIZUKA, M
中科院分区：
文献类型：
--
作者：
ARAI, Y;MURAKAMI, S;NISHIZUKA, M
围产期用雄激素处理雌性大鼠减少视前区前腹侧室周核（AVPvN-POA）的核体积。为了检查雄激素对神经发生的影响，在妊娠第15天（= E15）对同时接受丙酸睾酮（TP）注射的妊娠大鼠给予一次溴脱氧尿苷（BrdU）。在E17检查时，在AVPvN-POA中BrdU标记的神经元的数量在对照雌性、雄性和雄激素化雌性胎儿之间没有显著差异，这表明雄激素不干扰神经发生。在E21，在雄性和雄激素化雌性中观察到BrdU标记的AVPvN神经元显著减少。这些发现支持了这样的假设，即在雄性和雄激素化的雌性中，通过细胞死亡消除种群的能力增强。类似的选择性消除的AVPvN神经元发生在女性新生儿TP治疗。为了研究雄激素诱导的AVPvN-POA细胞死亡的性质，通过TdT介导的dUTP-生物素缺口末端标记（TUNEL）方法进行核DNA片段的特异性标记。TUNEL阳性细胞的数量显着更大的卵巢雄激素化的女性，与对照组女性相比。由于DNA断裂被认为是细胞凋亡的最典型特征，并且TUNEL方法是基于直接、特异性地原位标记细胞核中的DNA断裂，因此AVPvN-POA中的神经元死亡是凋亡性的，围产期雄激素可能诱导AVPvN-POA中的选择性凋亡性细胞死亡。(C)1994年出版社出版。
Perinatal treatment of female rats with andro en decreases the nuclear volume of the anteroventral periventricular nucleus of the preoptic area (AVPvN-POA). In order to examine the effect of androgen on neurogenesis, bromodeoxyuridine (BrdU) was given once on Day 15 of gestation (= E15) to pregnant rats that also received testosterone propionate (TP) injections. When examined at E17, the number of BrdU-labeled neurons in the AVPvN-POA was not significantly different among control female, male, and androgenized female fetuses, suggesting that androgen does not interfere with neurogenesis. At E21, a significant reduction of BrdU-labeled AVPvN neurons was observed in males and androgenized females. These findings support the hypothesis that elimination of a population by cell death is enhanced in males and androgenized females. Similar selective elimination of the AVPvN neurons occurred in the female following neonatal TP treatment. In order to investigate the nature of androgen-induced cell death in the AVPvN-POA, specific labeling of nuclear DNA fragmentation was performed by the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method. The number of TUNEL-positive cells was significantly greater in neonatally androgenized females, compared to that in control females. Since DNA fragmentation is considered the most characteristic feature of apoptosis, and TUNEL method is based on direct, specific labeling of DNA fragmentation in nuclei in situ, the neuronal death in the AVPvN-POA is apoptotic, and perinatal androgen may induce the selective apoptotic cell death in the AVPvN-POA. (C) 1994 Academic Press, Inc.