Novel method to observe subtle structural modulation of stratum corneum on applying chemical agents
Novel method to observe subtle structural modulation of stratum corneum on applying chemical agents
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DOI：
10.1016/j.chemphyslip.2010.02.005
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发表时间：
2010-05-01
影响因子：
3.4
通讯作者：
Yagi, Naoto
中科院分区：
文献类型：
--
作者：
Hatta, Ichiro;Nakazawa, Hiromitsu;Yagi, Naoto
在经皮渗透增强剂和化妆品等功能性化学品的开发中，角质层（SC）分子水平上的结构证据是非常必要的。我们开发了一种利用同步x射线衍射技术观察化学物质作用于SC时细胞间脂质基质和角质层的微小结构变化的方法。用氯仿/甲醇混合物、亲水性乙醇和疏水性d-柠檬烯等典型化学试剂验证了该方法的性能。从小角和广角x射线衍射中，我们得到了以下结果：在使用氯仿/甲醇混合物时，细胞间脂质被明显提取；在使用乙醇时，细胞间脂质结构被轻微破坏，乙醇分子被带入角质瘤细胞，此外乙醇池似乎在SC的细胞间脂质基质的亲水区域形成；在使用d-柠檬烯时，由于加入d-柠檬烯分子，长片层结构的重复距离增加。细胞间脂质结构被轻微破坏，并在细胞间脂质基质的疏水性区域形成d-柠檬烯池。(C) 2010 Elsevier Ireland Ltd.。版权所有。
In the development of functional chemicals such as percutaneous penetration enhancers and cosmetics, the structural evidence at the molecular level in stratum corneum (SC) is highly desirable. We developed a method to observe a minute structural change of intercellular lipid matrix and corneocytes on applying the chemicals to the SC using synchrotron X-ray diffraction technique. The performance of the present method was demonstrated by applying typical chemicals, chloroform/methanol mixture, hydrophilic ethanol and hydrophobic d-limonene. From the small- and wide-angle X-ray diffraction we obtained the following results: on applying chloroform/methanol mixture the intercellular lipids were extracted markedly, on applying ethanol the intercellular lipid structure was slightly disrupted, ethanol molecules were taken into the corneocytes and in addition the pools of ethanol seem to be formed in the hydrophilic region of the intercellular lipid matrix in the SC, and on applying d-limonene the repeat distance of the long lamellar structure increased by incorporating d-limonene molecules, the intercellular lipid structure was slightly disrupted, and the pools of d-limonene were formed in the hydrophobic region of the intercellular lipid matrix in the SC. (C) 2010 Elsevier Ireland Ltd. All rights reserved.