Non-invasive monitoring of hepatocellular carcinoma in transgenic mouse with bioluminescent imaging.
Non-invasive monitoring of hepatocellular carcinoma in transgenic mouse with bioluminescent imaging.
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DOI：
10.1016/j.canlet.2011.06.013
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发表时间：
2011-11
期刊：
影响因子：
9.7
通讯作者：
Ju Hui Park;K. Kim;Y. Lee;T. Lee;KyeongMin Kim;Sang-Soep Nahm;Young-Seo Park;G. Cheon;S. Lim;J. Kang
中科院分区：
文献类型：
--
作者：
Ju Hui Park;K. Kim;Y. Lee;T. Lee;KyeongMin Kim;Sang-Soep Nahm;Young-Seo Park;G. Cheon;S. Lim;J. Kang
一种小动物成像系统用于检测肝细胞癌(HCC)特异性报告基因的表达，将使体内监测癌变或治疗干预成为可能。建立了甲胎蛋白增强子/启动子调控萤火虫荧光素酶(Fluc)表达的转基因小鼠。转基因新生儿的肝脏区域有较强的生物发光信号，出生后发光信号减弱。用N-亚硝酸二乙胺处理的转基因小鼠的生物发光成像(BLI)显示，肝脏中有明显的flc活性，并随着时间的推移而增加。该转基因小鼠模型可用于BLI法监测一种化学致癌物在活体动物体内产生肝癌的AFP。
A small animal imaging system for hepatocellular carcinoma (HCC)-specific reporter gene expression will enable monitoring of carcinogenesis or therapeutic intervention in vivo. Transgenic mouse was developed in which firefly luciferase (fLuc) expression was controlled by the AFP enhancer/promoter. The bioluminescent signals of the transgenic neonates were strong at their liver region and decreased after birth. Bioluminescent imaging (BLI) of a transgenic mouse treated with N-nitrosodiethylamine revealed distinct fLuc activity in the liver and an increased pattern with time. The transgenic mouse model can be used to monitor AFP producing HCC by a chemical carcinogen in a live animal by BLI.