Noninvasive measurement of intestinal inflammation after burn injury.
Noninvasive measurement of intestinal inflammation after burn injury.
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DOI：
10.1097/bcr.0b013e318280e2f8
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发表时间：
2013-11
期刊：
影响因子：
--
通讯作者：
Choudhry MA
中科院分区：
文献类型：
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作者：
Sigman M;Conrad P;Rendon JL;Akhtar S;Eberhardt J;Gamelli RL;Choudhry MA
肠道炎症与烧伤和其他创伤性损伤中的多器官衰竭有关。我们假设肠道炎症的标志物是可检测的非侵入性。从7名严重烧伤患者和15名对照患者收集粪便样品，用于使用多重测定试剂盒（Bio-Rad）测量炎性细胞因子。此外，使用标准程序测量髓过氧化物酶（MPO）和弹性蛋白酶的粪便水平。与对照组相比，烧伤组的炎性细胞因子水平显著升高。IL-6升高2.16 ± 0.61pg/mg，（平均值± SEM）至3.81 ± 0.49; p<0.05，IL-8也是如此（3.32 ± 0.76 pg/mg至20.51 ± 6.65 p<0.05），IL-12（4.457 ± 0.98pg/mg至7.371 ± 0.95 p<0.05），IL-13（3.86 ± 0.32 pg/mg至11.83 ± 1.47 p<0.05），MCP-1（2.78 ± 2.61 pg/mg至6.5 ± 3.97 p<0.05）、MPO（13.41 ± 1.40单位/mg蛋白至24.52 ± 4.31 p<0.05）和弹性蛋白酶（2.46 ± 0.38 pg/ml至5.08 ± 0.72 p<0.05）。我们的研究结果表明，肠道炎症的标志物是可测量的非侵入性手段，并增加烧伤后相比，控制。值得注意的是，增加的IL-8与增加的MPO和弹性蛋白酶活性相关，表明中性粒细胞活化在烧伤介导的肠道炎症中的作用。因此，这些炎症细胞因子谱可能是烧伤后肠道炎症的有价值的生物标志物。
Intestinal inflammation has been linked with multi-organ failure in burn and other traumatic injuries. We hypothesized that markers of intestinal inflammation are detectible non-invasively. Fecal samples were collected from 7 severely burned patients and 15 control patients for the measurement of inflammatory cytokines using a multiplex assay kit (Bio-Rad). In addition, fecal levels of myeloperoxidase (MPO) and elastase were measured using standard procedures. Compared to a control group, levels of inflammatory cytokines were significantly increased in the burn group. IL-6 was increased 2.16 ± 0.61 pg/mg, (mean ± SEM) to 3.81 ± 0.49; p<0.05, as were IL-8 (3.32 ± 0.76 pg/mg to 20.51 ± 6.65 p<0.05), IL-12 (4.457 ± 0.98pg/mg to 7.371 ± 0.95 p<0.05), IL-13 (3.86 ± 0.32 pg/mg to 11.83 ± 1.47 p<0.05), MCP-1 (2.78 ± 2.61 pg/mg to 6.5 ± 3.97 p<0.05), MPO (13.41 ± 1.40 units/mg protein to 24.52 ± 4.31 p<0.05) and elastase (2.46 ± 0.38 pg/ml to 5.08 ± 0.72 p<0.05). Our results suggest that markers of intestinal inflammation are measurable by non-invasive means and are increased following burn injury compared to controls. Of note, increased IL-8 correlated with increased MPO and elastase activity, suggesting a role for neutrophil activation in burn-mediated intestinal inflammation. Thus, these inflammatory cytokine profiles may be valuable biomarkers of intestinal inflammation following burn injury.