REGENERATION OF TAIL BUD IN XENOPUS EMBRYOS
REGENERATION OF TAIL BUD IN XENOPUS EMBRYOS
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DOI：
10.1002/jez.1401920311
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发表时间：
1975-01-01
影响因子：
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通讯作者：
DEUCHAR, EM
中科院分区：
文献类型：
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作者：
DEUCHAR, EM
为了解决脊椎动物胚胎附肢再生能力这一长期存在的争议，对非洲爪哇胚胎的尾芽在26-32天的时间段进行了截尾，并对其在2-3天的培养期间再生的能力进行了研究。在断头期26-28，尾芽仅由未分化的中胚层和外胚层组成，而在32期，尾芽具有完全分化的神经管、液泡化的脊索和分段的体节。不同时期的137例截肢结果一致：所有手术的幼虫都形成了一条尾巴，大多数情况下它有正常的、发育良好的轴向组织。尾部结构异常的病例相对较少，为发育迟缓、水肿性幼虫，并伴有躯干部位的缺陷。从这些实验得出的结论是，即使在胚胎晚期，原始尾芽附近的细胞也能够分化为尾芽组织并取代被切断的区域。讨论了这些发现对脊椎动物再生的比较研究的意义。
In an attempt to solve some aspects of the long‐standing controversy about the regenerative ability of appendages in vertebrate embryos, the tail bud ofXenopus laevisembryos has been amputated at stages ranging from St. 26 to St. 32 and its ability to regenerate during a culture period of 2–3 days has been studied. At amputation stages 26–28, the tail bud consisted only of undifferentiated mesoderm and ectoderm, but at stage 32 it had a fully differentiated neural tube, a vacuolated notochord and segmented somites. A total of 137 amputations at different stages gave consistent results: a tail formed in all the operated larvae and it had normal, well‐developed axial tissues in most cases. The relatively few cases with abnormal tail structure were stunted, oedematous larvae with defects in the trunk region as well. It is concluded from these experiments that cells near the original tail bud are able to differentiate into tailbud tissue and to replace the amputated region, even at these late embryonic stages. The implications of these findings for comparative studies on regeneration in vertebrates are discussed.