Carbon-nanotube-interfaced glass fiber scaffold for regeneration of transected sciatic nerve
Carbon-nanotube-interfaced glass fiber scaffold for regeneration of transected sciatic nerve
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DOI：
10.1016/j.actbio.2014.11.026
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发表时间：
2015-02-01
影响因子：
9.7
通讯作者：
Hyun, Jung Keun
中科院分区：
文献类型：
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作者：
Ahn, Hong-Sun;Hwang, Ji-Young;Hyun, Jung Keun
碳纳米管（CNT），以其独特的和前所未有的性能，已成为非常受欢迎的组织修复，特别是对于那些需要电刺激。虽然大多数报告已经证明了CNT的体外神经细胞反应，但很少有研究表明CNT界面生物材料在神经组织修复和再生中的体内功效。因此，我们在这里报告的第一次在体内功能的CNT接口的神经导管的再生横断大鼠坐骨神经。将氨基化的碳纳米管化学连接到磷酸盐玻璃微纤维（PGFs）的表面，并将碳纳米管与磷酸盐玻璃微纤维（CNT-PGFs）连接，成功地将其置于三维聚（L/D-乳酸）（PLDLA）管中。一项体外研究证实，背根神经节的神经突沿排列的CNT-PGF活跃地沿着生长，并且CNT界面显著增加了最大神经突长度。将CNT-PCF神经导管植入横断大鼠坐骨神经的10 mm间隙中16周后，通过与CNT的接口连接，再生轴突穿过支架的数量、重新支配的肌肉的横截面积和电生理结果均显著改善。在横断大鼠坐骨神经的再生过程中使用CNT-界面支架的这种第一种体内效果强烈支持CNT-界面PGF在神经导管和外周神经组织之间的界面处的潜在用途。(C)2014 Acta Materialia Inc.爱思唯尔有限公司出版
Carbon nanotubes (CNTs), with their unique and unprecedented properties, have become very popular for the repair of tissues, particularly for those requiring electrical stimuli. Whilst most reports have demonstrated in vitro neural cell responses of the CNTs, few studies have been performed on the in vivo efficacy of CNT-interfaced biomaterials in the repair and regeneration of neural tissues. Thus, we report here for the first time the in vivo functions of CNT-interfaced nerve conduits in the regeneration of transected rat sciatic nerve. Aminated CNTs were chemically tethered onto the surface of aligned phosphate glass microfibers (PGFs) and CNT-interfaced PGFs (CNT-PGFs) were successfully placed into three-dimensional poly(L/D-lactic acid) (PLDLA) tubes. An in vitro study confirmed that neurites of dorsal root ganglion outgrew actively along the aligned CNT-PGFs and that the CNT interfacing significantly increased the maximal neurite length. Sixteen weeks after implantation of a CNT-PCF nerve conduit into the 10 mm gap of a transected rat sciatic nerve, the number of regenerating axons crossing the scaffold, the cross-sectional area of the re-innervated muscles and the electrophysiological findings were all significantly improved by the interfacing with CNTs. This first in vivo effect of using a CNT-interfaced scaffold in the regeneration process of a transected rat sciatic nerve strongly supports the potential use of CNT-interfaced PGFs at the interface between the nerve conduit and peripheral neural tissues. (C) 2014 Acta Materialia Inc. Published by Elsevier Ltd.