MASS-DISTRIBUTION OF A PROBABLE TAIL-LENGTH-DETERMINING PROTEIN IN BACTERIOPHAGE-T4
MASS-DISTRIBUTION OF A PROBABLE TAIL-LENGTH-DETERMINING PROTEIN IN BACTERIOPHAGE-T4
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DOI：
10.1073/pnas.82.16.5550
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
EISERLING, FA
中科院分区：
文献类型：
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作者：
DUDA, RL;WALL, JS;EISERLING, FA
未染色的冷冻干燥标本的暗场扫描透射电子显微镜照片的分析确定，完整的噬菌体T4尾管的内部含有额外的密度，这是在用3 M盐酸胍处理的人工排空的管中缺失的。尾管的质量为3.1 × 104。106道尔顿，中心通道直径为3.2 nm。密度数据的定量分析与中央通道中存在多达六条蛋白质分子链一致，所述蛋白质分子链可用作确定噬菌体尾部长度的模板或标尺结构，并且可与噬菌体DNA一起注射到细胞中。
Analysis of dark-field scanning transmission electron micrographs of unstained freeze-dried specimens established that the interior of the intact bacteriophage T4 tail tube contains extra density that is missing in tubes artificially emptied by treatment with 3 M guanidine hydrochloride. The mass of the tail tube is 3.1 .times. 106 daltons, and the central channel is 3.2 nm in diameter. Quantitative analysis of the density data is consistent with the presence of up to six strands of a protein molecule in the central channel that could serve as the template or ruler structure that determines the length of the bacteriophage tail and that could be injected into the cell with the phage DNA.