Recovery and amplification of placental RNA from dried maternal blood spots: utility for noninvasive prenatal diagnosis
Recovery and amplification of placental RNA from dried maternal blood spots: utility for noninvasive prenatal diagnosis
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DOI：
10.1016/s1472-6483(10)60645-1
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发表时间：
2006-10-01
影响因子：
4
通讯作者：
Jorgez, Carolina
中科院分区：
文献类型：
--
作者：
Jorgez, Carolina
利用血浆中循环游离 RNA 的方法可用于癌症和产前遗传分析。鉴于这些潜在的作用，我们研究了在室温下储存不同时间长度后的干燥母体血斑中检测胎盘特异性 RNA 的可行性。使用实时聚合酶链式反应 (PCR)，在室温下保存长达 4 周的妊娠早期和中期妊娠的 11 份干燥血液样本中，有 9 份显示出胎盘特异性 β-人绒毛膜促性腺激素转录物的阳性扩增。这项工作证明了使用干燥的母体血斑分离和扩增胎盘 mRNA 的可行性。随着胎儿和胎盘 RNA 标记物的发展，这种方法将可以简化产前基因诊断和妊娠相关并发症的样本收集、运输和储存。
Methods utilizing circulating cell-free RNA in plasma have clinical applications for cancer and prenatal genetic analysis. Given these potential roles, the feasibility of detecting placental specific RNA in dried maternal blood spots after storage at room temperature for varying lengths of time was investigated. Using real-time polymerase chain reaction (PCR), positive amplification of placental-specific beta-human chorionic gonadotrophin transcripts was demonstrated in nine of 11 dried blood samples from first and second trimester pregnancies stored at room temperature for up to 4 weeks. This work demonstrates feasibility in isolation and amplification of placental mRNA using dried maternal blood spots. With the development of fetal and placental RNA markers, this approach would allow simplified collection, transport, and storage of samples for prenatal genetic diagnosis and pregnancy related complications.