Screening natural antioxidants in peanut shell using DPPH-HPLC-DAD-TOF/MS methods
Screening natural antioxidants in peanut shell using DPPH-HPLC-DAD-TOF/MS methods
复制标题
DOI：
10.1016/j.foodchem.2012.07.042
复制
发表时间：
2012-12-15
期刊：
影响因子：
8.8
通讯作者：
Du, Fangling
中科院分区：
文献类型：
--
作者：
Qiu, Jiying;Chen, Leilei;Du, Fangling
花生壳是石油生产的副产品，富含天然抗氧化剂。本文首次采用DPPH-HPLC-DAD-TOF/MS快速、高效地筛选花生壳中抗氧化剂。该方法基于与1，1-二苯基-2-苦肼基（DPPH）反应后，具有潜在抗氧化活性的化合物在HPLC色谱图中的峰面积将显著减少或消失的假设，并可通过HPLC-DAD-TOF/MS技术进行鉴别确认。结果表明，花生壳甲醇提取物中含有丰富的三种具有潜在抗氧化活性的化合物。经鉴定为5，7-二羟基色酮、圣草酚和木犀草素。这些化合物的含量分别为0.59、0.92和2.36 mg/g，其中木樨草素具有最强的自由基清除能力。DPPH-HPLC-DAD-TOF/MS法可快速鉴定和测定花生壳中的天然抗氧化剂，为花生加工副产物的增值利用提供参考。(C)2012爱思唯尔有限公司保留所有权利。
Peanut shell, a byproduct in oil production, is rich in natural antioxidants. Here, a rapid and efficient method using DPPH-HPLC-DAD-TOF/MS was used for the first time to screen antioxidants in peanut shell. The method is based on the hypothesis that upon reaction with 1,1-diphenyl-2-picrylhydrazyl (DPPH), the peak areas of compounds with potential antioxidant activities in the HPLC chromatogram will be significantly reduced or disappeared, and the identity confirmation could be achieved by HPLC-DAD-TOF/MS technique. With this method, three compounds possessing potential antioxidant activities were found abundantly in the methanolic extract of peanut shell. They were identified as 5,7-dihydroxychromone, eriodictyol, and luteolin. The contents of these compounds were 0.59, 0.92, and 2.36 mg/g, respectively, and luteolin possessed the strongest radical scavenging capacity. DPPH-HPLC-DAD-TOF/MS assay facilitated rapid identification and determination of natural antioxidants in peanut shell, which may be helpful for value-added utilization of peanut processing byproducts. (C) 2012 Elsevier Ltd. All rights reserved.