GENETIC COMPARISONS OF FRESHLY ISOLATED VERSUS CULTURED SYMBIOTIC DINOFLAGELLATES: IMPLICATIONS FOR EXTRAPOLATING TO THE INTACT SYMBIOSIS
GENETIC COMPARISONS OF FRESHLY ISOLATED VERSUS CULTURED SYMBIOTIC DINOFLAGELLATES: IMPLICATIONS FOR EXTRAPOLATING TO THE INTACT SYMBIOSIS
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新鲜分离与培养的共生甲藻的遗传比较：外推完整共生的意义
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
M. Coffroth
中科院分区：
文献类型：
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作者：
S. R. Santos;D. J. Taylor;M. Coffroth
虫黄藻是不同海洋无脊椎动物宿主中的藻类共生体，是一个遗传异质的类群。虫黄藻的所有物种描述和大多数生理和感染性研究都是使用培养材料进行的。然而，很少有研究试图量化的代表性，从刺胞动物中分离出的寄生虫种群的个别主机或主机物种，他们建立的文化。进行了小亚基（18 S）rDNA的RFLP、内部转录间隔区（ITS）-rDNA序列数据和微卫星分析，以评估培养的虫黄藻与分离出的单个宿主或宿主物种的多孢子虫种群之间的相关性。RFLP数据表明，文化可能代表数量占优势的共生体或存在于较低的数字。虫黄藻培养物的ITS-rDNA序列与从同一宿主种的亚磷酸酯虫黄藻中鉴定的ITS-rDNA序列不一致，并且亚磷酸酯虫黄藻种群中存在的微卫星在相应的培养物中不存在。最后，重新审查的文献显示的例子zooxanthella文化是不具代表性的，在hospite人口。这些数据表明，在大多数情况下，文化是一个子集的原始在hospite人口。转移前未将大量培养物均质化、使用生长培养基和挑选单个运动细胞等因素可能导致许多虫黄藻培养物不具有代表性。
Zooxanthellae, algal symbionts in divergent marine invertebrate hosts, are a genetically heterogeneous group. All species descriptions and most physiological and infectivity studies of zooxanthellae have been conducted using cultured material. However, few studies have attempted to quantify the representation of cultures isolated from cnidarians to the in hospite zooxanthella populations of the individual host or host species from which they were established. RFLPs of small subunit (18S) rDNA, internal transcribed spacer (ITS)‐rDNA sequence data, and microsatellite analyses were conducted to assess the relatedness between cultured zooxanthellae and the in hospite populations of the individual host or host species from which they were isolated. RFLP data demonstrated that cultures may represent either the numerically dominant symbiont or ones present in lower number. ITS‐rDNA sequences from zooxanthella cultures were disconcordant with ITS‐rDNA sequences identified from in hospite zooxanthellae of the same host species, and microsatellites present in in hospite zooxanthella populations were absent from the corresponding cultures. Finally, reexamination of the literature revealed examples of zooxanthella cultures being nonrepresentative of in hospite populations. These data suggest that, in most cases, cultures are a subset of the original in hospite population. Factors such as failing to homogenize bulk cultures before transfer, growth medium used, and the picking of single motile cells may contribute to many zooxanthella cultures being nonrepresentative.