MULTIPESTICIDE RESIDUE METHOD FOR FRUITS AND VEGETABLES - CALIFORNIA-DEPARTMENT-OF-FOOD-AND-AGRICULTURE
MULTIPESTICIDE RESIDUE METHOD FOR FRUITS AND VEGETABLES - CALIFORNIA-DEPARTMENT-OF-FOOD-AND-AGRICULTURE
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DOI：
10.1007/bf00322352
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发表时间：
1991-01-01
期刊：
影响因子：
--
通讯作者：
CARR, JE
中科院分区：
文献类型：
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作者：
LEE, SM;PAPATHAKIS, ML;CARR, JE
本程序描述了用于新鲜水果和蔬菜中多种农药残留分析的CDFA方法。 将一等份切碎的样品与乙腈混合。 过滤所得乙腈水溶液提取物，并通过反相固相萃取装置进行净化。 使用磷酸盐缓冲液将滤液的pH值调节至中性，并通过“盐析”方法将乙腈层与水层分离。 用K-D蒸发器通过与正己烷形成共沸物来浓缩乙腈层的等分试样。 使用GLC和HPLC测定制备的样品的农药残留。 用0.1- 0.2ppm的7种氯代烃、7种有机磷和7种N-甲基氨基甲酸酯类农药对6种有代表性的水果和蔬菜进行了强化检测。 未观察到基质干扰，不同样品以及不同农药的残留回收率不同。 百菌清结果差异很大，回收率不可重现。 一般而言，该方法似乎快速、耐用，并且能够在0.01 ppm水平下进行常规检测。
This procedure describes the CDFA method for multipesticide residue analysis of fresh fruits and vegetables. An aliquot of chopped sample is blended with acetonitrile. The resulting aqueous acetonitrile extract is filtered and cleaned up via reverse phase solid phase extraction apparatus. The pH of the filtrate is adjusted to neutral using phosphate buffer and the acetonitrile layer is separated from the aqueous layer by a "salting out" process. An aliquot of the acetonitrile layer is concentrated with a K-D evaporator by forming an azeotrope with n-hexane. The prepared sample is assayed for pesticide residues using GLC and HPLC. The performance of this method was evaluated by fortifying 6 representative fruits and vegetables with 7 chlorinated hydrocarbons, 7 organophosphate and 7 N-methylcarbamate pesticides at 0.1-0.2 ppm. No matrix interference was observed and the recovery of residues varied among different samples as well as different pesticides. Chlorothalonil results varied widely with irreproducible recoveries. In general, the method appears to be fast, rugged, and able to detect routinely at the 0.01 ppm level.