Painful nerve injury upregulates thrombospondin-4 expression in dorsal root ganglia.
Painful nerve injury upregulates thrombospondin-4 expression in dorsal root ganglia.
复制标题
疼痛神经损伤上调背根神经节中血小板反应蛋白 4 的表达。
DOI：
10.1002/jnr.23498
复制
发表时间：
2015
影响因子：
4.2
通讯作者：
Hogan,QuinnH
中科院分区：
文献类型：
--
作者：
Pan,Bin;Yu,Hongwei;Park,John;Yu,YanhuiPeter;Luo,ZDavid;Hogan,QuinnH
血小板反应蛋白-4 (TSP4) 属于一个大的寡聚细胞外基质糖蛋白家族，介导细胞之间的相互作用以及细胞与底层基质成分的相互作用。最近的证据表明 TSP4 可能会导致神经性疼痛的产生。然而，尚未对损伤后背根神经节（DRG）中 TSP4 的表达进行系统检查。因此，本研究通过蛋白质印迹、免疫组织化学和免疫细胞化学研究脊髓神经结扎（SNL）损伤后大鼠背根神经节中TSP4蛋白水平是否发生变化，并避免神经损伤。神经结扎后，第五腰椎 (L5) DRG 的轴突体中 TSP4 蛋白水平上调。 L5 DRG 的非神经元区室中存在大量额外的 TSP4，其不包含卫星胶质细胞、小胶质细胞或雪旺细胞的标记，并且似乎位于间隙空间中。 SNL 后从 L5 DRG 分离的神经元中持续存在 TSP4 细胞内过度表达的证据。这些发现表明，周围神经损伤后，轴突感觉神经元的细胞质和周围的间隙空间中 TSP4 蛋白表达升高。 © 2014 Wiley 期刊公司。
Thrombospondin‐4 (TSP4) belongs to a family of large, oligomeric extracellular matrix glycoproteins that mediate interactions between cells and interactions of cells with underlying matrix components. Recent evidence shows that TSP4 might contribute to the generation of neuropathic pain. However, there has been no systematic examination of TSP4 expression in the dorsal root ganglia (DRG) after injury. This study, therefore, investigates whether TSP4 protein level is changed in DRG after injury following spinal nerve ligation (SNL) and spared nerve injury in rats by performing Western blotting, immunohistochemistry, and immunocytochemistry. After nerve ligation, TSP4 protein level is upregulated in the axotomized somata of the fifth lumbar (L5) DRG. There is substantial additional TSP4 in the nonneuronal compartment of the L5 DRG that does not costain for markers of satellite glia, microglia, or Schwann cells and appears to be in the interstitial space. Evidence of intracellular overexpression of TSP4 persists in neurons dissociated from the L5 DRG after SNL. These findings indicate that, following peripheral nerve injury, TSP4 protein expression is elevated in the cytoplasm of axotomized sensory neurons and in the surrounding interstitial space. © 2014 Wiley Periodicals, Inc.