Capillary-based displacement flow immunosensor
Capillary-based displacement flow immunosensor
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DOI：
10.1021/ac961037y
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发表时间：
1997-05-15
影响因子：
7.4
通讯作者：
Ligler, FS
中科院分区：
文献类型：
--
作者：
Narang, U;Gauger, PR;Ligler, FS
本文报道了一种新型的2，4，6-三硝基甲苯（TNT）毛细管流动免疫传感器的研制。将抗TNT抗体固定在800 μ m-i. d.使用异双功能交联剂的玻璃毛细管，随后用荧光团标记的抗原使毛细管饱和。为了进行测定，将TNT溶液的等分试样注入毛细管中。TNT竞争的荧光团标记的抗原，这是下游检测的结合口袋，我们已经证明，毛细管免疫传感器的灵敏度提高了至少2个数量级相比，传统的填充柱;或基于膜的位移流免疫传感器。毛细管免疫传感器对TNT的检测限（S/N > 3）为15 pg/mL（7 fmol），线性动态范围为1-250 ng/mL。整个分析可以在不到3分钟内进行。在15次100 ng/mL TNT溶液进样期间，信号重现性（RSD）保持在4%以内。
We report on the preparation of a novel capillary flow immunosensor for 2,4,6-trinitrotoluene (TNT). Anti-TNT antibody is immobilized onto the silanized inner walls of an 800-mu m-i.d. glass capillary using a heterobifunctional crosslinker, followed by saturating the capillary with fluorophore-labeled antigen. To perform the assay, an aliquot of TNT solution is injected into the capillary. TNT competes for the binding pocket of the fluorophore-labeled antigen, which is detected downstream, We have demonstrated that the sensitivity of the capillary immunosensor is improved by at least 2 orders of magnitude compared to that of conventional packed-column;or membrane-based displacement flow immunosensors. The limit of detection (S/N > 3) for TNT using the capillary immunosensor is 15 pg/mL (7 fmol), and the linear dynamic range is 1-250 ng/mL. The entire assay can be performed in less than 3 min. During 15 injections of 100 ng/mL TNT solution, the signal reproducibility (RSD) was maintained to within 4%.