DNA specificity determinants of Escherichia coli tryptophan repressor binding.
DNA specificity determinants of Escherichia coli tryptophan repressor binding.
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大肠杆菌色氨酸阻遏物结合的 DNA 特异性决定因素。
DOI：
10.1101/gad.1.6.565
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发表时间：
1987
影响因子：
10.5
通讯作者：
Youderian,P
中科院分区：
文献类型：
--
作者：
Bass,S;Sugiono,P;Arvidson,DN;Gunsalus,RP;Youderian,P
我们已经分析了大肠杆菌色氨酸（Trp）阻遏物和它的运营商使用挑战噬菌体载体之间的序列特异性相互作用。沙门氏菌噬菌体P22的衍生物，这些衍生物用合成的对称trp操纵子取代了P22 mnt操纵子，提供了体内DNA结合的遗传测定。携带保留Trp阻遏物结合决定簇的操纵子的噬菌体有效地使产生阻遏物的细胞溶原化;相反，携带缺少关键决定簇的操纵子的噬菌体杀死这样的宿主。该试验揭示的结合决定簇证实了从阻遏物晶体结构假设的色氨酸阻遏物-操纵子相互作用的简单对接模型，并解释了阻遏物结合的特异性和色氨酸阻遏物识别多个串联DNA位点的能力。
We have analyzed the sequence-specific interaction between the Escherichia coli tryptophan (Trp) repressor and its operator using challenge phage vectors. These phages, derivatives of Salmonella phage P22 that have substitutions of synthetic, symmetric trp operators for the P22 mnt operator, provide a genetic assay for DNA binding in vivo. Phages carrying operators that retain the determinants of Trp repressor binding efficiently lysogenize cells producing repressor; in contrast, phages with operators missing critical determinants kill such hosts. The binding determinants revealed by this assay corroborate a simple docking model for the Trp repressor-operator interaction postulated from the repressor crystal structure, and account for both the specificity of repressor binding and the ability of Trp repressor to recognize multiple, tandem DNA sites.