Root growth angle: An important trait that influences the deep rooting of apple rootstocks
Root growth angle: An important trait that influences the deep rooting of apple rootstocks
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DOI：
10.1016/j.scienta.2017.01.019
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发表时间：
2017-02-14
影响因子：
4.3
通讯作者：
Han, Zhenhai
中科院分区：
文献类型：
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作者：
An, Haishan;Dong, Haiqiang;Han, Zhenhai
根生长角（RGA）是决定植物根系发育的主要形态性状之一。然而，迄今为止，还没有关于苹果RGA的遗传信息。本研究以深根巴棱蟹（BC）、浅根M9和BC × M9杂交的115个种内杂种后代为材料，对RGA的表型变异进行了研究。生长素（IAA）的含量和表达的基因，控制RGA和根向地性，MdDROS和MdPIN 11，也进行了分析。结果表明，M9的RGA小于巴棱蟹。M9的根中IAA水平和MdDRO1、MdPIN11的表达量均显著低于巴棱蟹，表明生长素信号和这两个基因在RGA调控中的潜在重要性。此外，M9的根冠中的柱状细胞中淀粉体数量少于巴棱蟹。RGA和根长在杂种后代中均表现出正态分布和超亲分离，表明这两个性状本质上都是数量性状，受多基因控制。RGA和根长都是影响生根的重要性状。这些可能受到MdDRO 1和MdPIN 11的影响。(C)2017爱思唯尔B.V.保留所有权利。
Root growth angle (RGA) is one of the major morphological traits that determine whether a plant develops a shallow or deep root system. To date, however, no genetic information has been available regarding RGA in apple (Mains domestica Borkh.). In this study, phenotypic variation in RGA was investigated using deep-rooted Baleng Crab (BC), shallow-rooted M9, and 115 intraspecific hybrid progenies derived from a BC x M9 cross. Auxin (IAA) content and the expression of genes that control RGA and root gravitropism, MdDROs and MdPIN11, were also analyzed. The results revealed that M9 had a smaller RGA than Baleng Crab. The IAA level and the expression of both MdDRO1 and MdPIN11 were significantly lower in the roots of M9 than in those of Baleng Crab, indicating the potential importance of auxin signaling and these two genes in the control of RGA. Furthermore, fewer amyloplasts were detected in columella cells in the root cap of M9 in comparison with Baleng Crab. Both RGA and root length showed a normal distribution and transgressive segregation in hybrid progeny, suggesting that both these traits are quantitative in nature and controlled by polygenes. We conclude that both RGA and root length are important traits contributing to deep rooting in M. domestica and that these might be influenced by MdDRO1 and MdPIN11. (C) 2017 Elsevier B.V. All rights reserved.