Effect of macromer weight percent on neural cell growth in 2D and 3D nondegradable PEG hydrogel culture
Effect of macromer weight percent on neural cell growth in 2D and 3D nondegradable PEG hydrogel culture
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DOI：
10.1002/jbm.a.32787
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发表时间：
2010-09-15
影响因子：
4.9
通讯作者：
Mahoney, Melissa J.
中科院分区：
文献类型：
--
作者：
Lampe, Kyle J.;Mooney, Rachael G.;Mahoney, Melissa J.
神经前体细胞（NPC）是一种可再生细胞来源，可用于神经细胞移植疗法。传统上通过在硬质组织培养聚苯乙烯上培养来探索它们的扩张和分化潜力。在这里，我们描述了替代培养系统的优点：生物惰性聚乙二醇（PEG）水凝胶。具体而言，这项工作报告了大分子单体重量百分比对在 2D 和 3D PEG 水凝胶内生长的神经元和多能 NPC 混合群的代谢和细胞凋亡活性、增殖和细胞组成的影响。在 2D 培养中，水凝胶特性不会影响代谢或细胞凋亡活性，但会影响细胞增殖和组成，导致神经胶质细胞反应性随着硬度的增加而增加。在 3D 培养中，低重量百分比的水凝胶导致更高的代谢活性和更低的细胞凋亡活性，并且仅在与天然脑组织的硬度密切匹配的水凝胶中观察到显着的增殖。因此，PEG水凝胶提供了一种多功能的体外培养系统，只需改变水凝胶的材料特性，即可用于培养和扩增各种神经细胞和神经胶质细胞类型。 (C) 2010 Wiley periodicals, Inc. J Biomed Mater Res Part A：94A：1162-1171，2010。
Neural precursor cells (NPCs) are a renewable cell source that may be useful for neural cell transplant therapies. Their expansion and differentiation potential have traditionally been explored by culturing them on stiff tissue culture polystyrene. Here we describe advantages of an alternative culture system: bio-inert poly(ethylene glycol) (PEG) hydrogels. Specifically this work reports the effect that macromer weight percent has on the metabolic and apoptotic activity, proliferation, and cellular composition of a mixed population of neurons and multipotent NPCs grown both on 2D and within 3D PEG hydrogels. In 2D culture, hydrogel properties did not affect metabolic or apoptotic activity but did impact cell proliferation and composition leading to an increase in glial cell reactivity as stiffness increased. In 3D culture, low weight percent hydrogels led to greater metabolic activity and lower apoptotic activity with significant proliferation observed only in hydrogels that closely matched the stiffness of native brain tissue. PEG hydrogels therefore provide a versatile in vitro culture system that can be used to culture and expand a variety of neural and glial cell types simply by altering the material properties of the hydrogel. (C) 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 94A: 1162-1171, 2010.