Cytochrome bc1 Complex: Potential Breach to Improve the Activity of Phenazines on Xanthomonas.
Cytochrome bc1 Complex: Potential Breach to Improve the Activity of Phenazines on Xanthomonas.
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DOI：
10.1021/acs.jafc.2c03317
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发表时间：
2022-08
影响因子：
6.1
通讯作者：
Jian Wu;Qingzhu Teng;Y. Mao;Y. Duan;Xiayan Pan;Shu Xu;Yiqiang Cai;Yuemin Pan;Mingguo Zhou;Yong Zhang
中科院分区：
文献类型：
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作者：
Jian Wu;Qingzhu Teng;Y. Mao;Y. Duan;Xiayan Pan;Shu Xu;Yiqiang Cai;Yuemin Pan;Mingguo Zhou;Yong Zhang
据报道，天然杀虫剂吩嗪的作用受到黄单胞菌内某些耐受代谢过程的限制。我们之前的研究表明，功能性细胞色素 bc1 复合物是呼吸链中不可或缺的组成部分，可能参与黄单胞菌对吩嗪的耐受。在本研究中，野油菜黄单胞菌 pv. 的细胞色素 bc1 突变体。 Campestris (Xcc) 和 Xanthomonas oryzae pv.构建了对吩嗪表现出不同耐受能力的米菌 (Xoo)，并且细胞色素 bc1 复合物被证明在黄单胞菌耐受吩嗪方面发挥着关键且保守的作用。此外，细胞色素c突变体的结果表明黄单胞菌中各种细胞色素c蛋白具有不同的功能，并且细胞色素bc1复合物向细胞色素C4传导的电子是揭示耐受机制的关键。总之，细胞色素 bc1 复合物的研究提供了提高吩嗪抗黄单胞菌活性的潜在策略。
The effects of the natural pesticides, phenazines, were reported to be limited by some tolerant metabolism processes within Xanthomonas. Our previous studies suggested that the functional cytochrome bc1 complex, the indispensable component of the respiration chain, might participate in tolerating phenazines in Xanthomonas. In this study, the cytochrome bc1 mutants of Xanthomonas campestris pv. campestris (Xcc) and Xanthomonas oryzae pv. oryzae (Xoo), which exhibit different tolerance abilities to phenazines, were constructed, and the cytochrome bc1 complex was proven to partake a critical and conserved role in tolerating phenazines in Xanthomonas. In addition, results of the cytochrome c mutants suggested the different functions of the various cytochrome c proteins in Xanthomonas and that the electron channeled by the cytochrome bc1 complex to cytochrome C4 is the key to reveal the tolerance mechanism. In conclusion, the study of the cytochrome bc1 complex provides a potential strategy to improve the activity of phenazines against Xanthomonas.