Proteomic Analysis of Exosomes from Adipose-Derived Mesenchymal Stem Cells: A Novel Therapeutic Strategy for Tissue Injury
Proteomic Analysis of Exosomes from Adipose-Derived Mesenchymal Stem Cells: A Novel Therapeutic Strategy for Tissue Injury
复制标题
脂肪源性间充质干细胞外泌体的蛋白质组学分析：组织损伤的新型治疗策略。
DOI：
10.1155/2020/6094562
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发表时间：
2020-03-04
影响因子：
--
通讯作者：
Li, Zubing
中科院分区：
文献类型：
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作者：
Xing, Xin;Han, Shuang;Li, Zubing
胞外体是细胞外的膜性纳米囊泡，通过将蛋白质等功能分子输送到靶细胞，从而影响受体细胞的行为，从而介导局部和系统的细胞间通讯。来源于脂肪来源的间充质干细胞(ADSCs)的外体被认为是一种多功能且丰富的组织损伤治疗工具。为了研究ADSC分泌的外切体及其在组织修复中的潜在功能，我们通过超速离心法从ADSC的上清液中分离出外切体，并通过透射电子显微镜、纳米颗粒跟踪分析和Western印迹分析对其进行了表征。然后，我们通过蛋白质组学分析确定了它们的蛋白质谱。结果表明，胞外囊泡的平均直径为116 nm，呈杯状形态，表达胞外标志。在外切体中共鉴定出1185个蛋白质组。基因本体论分析表明，胞外体蛋白主要来源于参与蛋白质结合的细胞。通过同源基团簇系统对蛋白质的注释表明，大多数蛋白质参与一般功能预测、翻译后修饰、蛋白质周转和伴侣。进一步的途径分析表明，所获得的大部分蛋白质参与了代谢途径、局部黏附、肌动蛋白细胞骨架的调节以及微生物的代谢。一些与组织修复相关的信号通路也被发现。识别出的分子可能成为未来研究的潜在治疗靶点。
Exosomes are extracellular membranous nanovesicles that mediate local and systemic cell-to-cell communication by transporting functional molecules, such as proteins, into target cells, thereby affecting the behavior of receptor cells. Exosomes originating from adipose-derived mesenchymal stem cells (ADSCs) are considered a multipotent and abundant therapeutic tool for tissue injury. To investigate ADSC-secreted exosomes and their potential function in tissue repair, we isolated exosomes from the supernatants of ADSCs via ultracentrifugation, characterized them via transmission electron microscopy, nanoparticle tracking analysis, and Western blot analysis. Then, we determined their protein profile via proteomic analysis. Results showed that extracellular vesicles, which have an average diameter of 116 nm, exhibit a cup-shaped morphology and express exosomal markers. A total of 1,185 protein groups were identified in the exosomes. Gene Ontology analysis indicated that exosomal proteins are mostly derived from cells mainly involved in protein binding. Protein annotation via the Cluster of Orthologous Groups system indicated that most proteins were involved in general function prediction, posttranslational modification, protein turnover, and chaperoning. Further, pathway analysis revealed that most of the proteins obtained participated in metabolic pathways, focal adhesion, regulation of the actin cytoskeleton, and microbial metabolism. Some tissue repair-related signaling pathways were also discovered. The identified molecules might serve as potential therapeutic targets for future studies.