COMPUTER-ASSISTED METHOD FOR DETERMINATION OF RESTRICTION ENZYME RECOGNITION SITES
COMPUTER-ASSISTED METHOD FOR DETERMINATION OF RESTRICTION ENZYME RECOGNITION SITES
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DOI：
10.1093/nar/5.11.4105
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发表时间：
1978-01-01
影响因子：
14.9
通讯作者：
ROBERTS, RJ
中科院分区：
文献类型：
--
作者：
GINGERAS, TR;MILAZZO, JP;ROBERTS, RJ
已经开发了一种计算机程序，其有助于确定限制性内切酶识别序列。这是通过用限制性内切酶切割已知序列的DNA并将片断模式与计算机生成的模式集进行比较来实现的。这种方法的可行性已经用已知和未知特异性的酶产生的øx174 DNA的片段模式进行了测试。使用该方法对两种限制性内切酶(BbvI和SfaNI)的识别序列进行了预测。此外，使用另一种计算机辅助方法预测了另外两种新酶(PvuI和MstI)的识别序列。
A computer program has been developed which aids in the determination of restriction enzyme recognition sequences. This is achieved by cleaving DNAs of known sequence with a restriction endonuclease and comparing the fragmentation pattern with a computer-generated set of patterns. The feasibility of this approach has been tested using fragmentation patterns of øX174 DNA produced by enzymes of both known and unknown specificity. Recognition sequences are predicted for two restriction endonucleases (BbvIandSfaNI) using this method. In addition, recognition sequences are predicted for two other new enzymes (PvuIandMstI) using another computer-assisted method.