Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling
Detection of apoptosis induced by new type gosling viral enteritis virus in vitro through fluorescein annexin V-FITC/PI double labeling
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DOI：
10.3748/wjg.14.2174
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发表时间：
2008-04-14
影响因子：
4.3
通讯作者：
Peng, Xi
中科院分区：
文献类型：
--
作者：
Chen, Shun;Cheng, An-Chun;Peng, Xi
目的：为深入了解新型雏鹅病毒性肠炎病毒（NGVEV）的致病机理及其与宿主细胞的关系，采用Annexin V-FITC和PI染色，流式细胞仪和荧光显微镜观察NGVEV诱导鸭胚成纤维细胞（DEF）凋亡的情况。通过用荧光素膜联蛋白V-FITC和PI的组合染色细胞，可以区分和定量分析非凋亡细胞（膜联蛋白V-FITC阴性/PI阴性），早期凋亡细胞（膜联蛋白V-FITC阳性/PI阴性），流式细胞术和荧光显微镜观察晚期凋亡/坏死细胞（Annexin V-FITC阳性/PI阳性）和死亡细胞（Annexin V-FITC阴性/PI阳性）。结论：NGVEV能诱导感染的DEF细胞发生凋亡，且凋亡先于坏死。(C)2008年WJG。All rights reserved.
AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells.METHODS: The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI).RESULTS: By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non-apoptotic cells decreased.CONCLUSION: NGVEV can induce the infected DEF cells to undergo apoptosis and the apoptosis occurs prior to necrosis. (C) 2008 WJG. All rights reserved.