Visualizing water-conduction pathways of living trees: selection of dyes and tissue preparation methods
Visualizing water-conduction pathways of living trees: selection of dyes and tissue preparation methods
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DOI：
10.1093/treephys/25.3.269
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发表时间：
2005-03-01
期刊：
影响因子：
4
通讯作者：
Utsumi, Y
中科院分区：
文献类型：
--
作者：
Sano, Y;Okamura, Y;Utsumi, Y
为了使活体树木的水传导途径可视化，我们将番红和酸性品红的水溶液引入到胡杨的茎中。为了研究每种染料在树木中的传播，我们比较了几种用于制备光学显微镜组织的技术。酸性品红比番红分布得更快、更广，反映了染料分子电离状态的差异。我们制备了一些切片，在通过冷冻干燥使染料稳定后，不允许染料再溶解。在这些切片中，在血管和一些相邻的射线薄壁细胞中观察到染料。在不稳定染料的情况下制备其他切片。在这些切片中，树液流中的酸性品红使细胞壁未染色，而番红使导管附近的木纤维以及导管本身染色，条件是将切片固定在甘油中，甘油溶解番红。虽然染色番红，木材纤维不含水。结果表明，引入的染料的稳定化和随后在避免染料再溶解的条件下制备的组织允许在细胞水平上准确地可视化水传导途径。
To visualize water-conduction pathways in living trees, we introduced aqueous solutions of safranin and acid fuchsin into stems of Populus sieboldii Miquel. To examine the spread of each dye in the trees, we compared several techniques for preparing tissue for light microscopy. Acid fuchsin was distributed more rapidly and more widely than safranin, reflecting differences between the dye molecules in state of ionization. We prepared some sections without allowing the dye to redissolve after it had been stabilized by freeze-drying. In these sections, the dye was observed in vessels and in some of the adjacent ray parenchyma cells. Other sections were prepared without stabilizing the dye. In these sections, acid fuchsin in the sap stream left cell walls unstained, whereas safranin stained wood fibers in the vicinity of vessels, as well as the vessels themselves, provided that the sections were mounted in glycerin, which dissolves safranin. Although stained with safranin, the wood fibers contained no water. The results indicate that stabilization of the introduced dye and subsequent preparation of tissues under conditions that avoid dye resolublilization allow accurate visualization of water-conduction pathways at the cellular level.